Anti hif 1α antibody

EnVision™ kits, horseradish-peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) and goat anti-rabbit IgG were obtained from Dako/Agilent (Santa Clara, CA, USA). A polyclonal anti-HIF-1α antibody was from Cayman (Ann Arbor, MI, USA). Anti-HIF-2 and anti-HIF-1β antibodies were obtained from Novus Biologicals (Litteton, CO, USA), and anti-LDH-A antibody was from Proteintech (Rosemont, IL, USA). Dulbecco’s modified Eagle’s medium (DMEM) with pyruvate and IFN-γ were obtained from Wako Pure Chemical Industries Ltd (Osaka, Japan) and DMEM without pyruvate was obtained from Nacalai Tesque (Kyoto, Japan). Fetal bovine serum (FBS) was from Equitech Bio (Kerrville, TX, USA). An anti-β-actin antibody and oxamate were purchased from Sigma (St Louis, MO, USA). Isogen was obtained from Nippon Gene (Toyama, Japan) and the ReverTra Ace qPCR RT Kit was from Toyobo (Osaka, Japan). The Ambion PARIS™ Kit and TaqMan gene expression master mix were purchased from Applied Biosystems (Carlsbad, CA, USA). SYBR Green real-time PCR master mix was purchased from Roche/Nippon Genetics (Tokyo, Japan). [¹⁴C]-Pyruvate was obtained from the Japan Radioisotope Association (Tokyo, Japan).