The extracted cell lysate was incubated with specific antibodies (anti-Ago2/IgG, 1 : 500, Abcam) overnight at 4°C and then pulled down with Protein G-Sepharose beads (Abcam). The beads were washed with lysis buffer five times and then digested with Proteinase K (Sigma‐Aldrich) for 1.5 hours. The digested solution underwent RNA purification using TRIzol Reagent. Quantitative RT-PCR was performed to examine the RNA yield. The primers used for analysis are listed below in the qRT-PCR section.
Anti ago2 igg
Manufactured by Abcam
Anti-Ago2/IgG is a laboratory product that detects the Argonaute 2 (Ago2) protein. Ago2 is a key component of the RNA-induced silencing complex (RISC) and plays a crucial role in microRNA-mediated gene silencing. This product is designed for research use only and its core function is to identify and quantify Ago2 levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti ago2 igg
1
Immunoprecipitation and RNA Quantification
2
Immunoprecipitation and RNA Analysis
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The OS cells were lysed and harvested using homemade lysis buffer (50 mmol/L Tris‐HCl, 150 mmol/L NaCl, 2 mmol/L EDTA, 1% Triton X‐100, 1% sodium deoxycholate) plus RNase and protease inhibitors (Roche). The extracted lysate was incubated with specific antibodies (anti–Ago2/IgG, 1:500, Abcam) overnight at 4°C and then pulled down with Protein G‐Sepharose beads (Abcam). The beads were washed with lysis buffer five times and then digested with Proteinase K (Sigma‐Aldrich) for 1.5 hours. The digested solution underwent RNA purification using TRIzol Reagent. Quantitative RT‐PCR was performed to examine the RNA yield. The primers used for analysis are listed below in the qRT‐PCR section.
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