Total RNA was isolated from GALA II subject nasal airway epithelial brushings using the AllPrep DNA/RNA Mini Kit (QIAGEN, Germantown, MD). Whole transcriptome libraries were constructed using the KAPA Stranded mRNA-seq library kit (Roche Sequencing and Life Science, Kapa Biosystems, Wilmington, MA) from 250ng of total input RNA with the Beckman Coulter Biomek FXP automation system (Beckman Coulter, Fullerton, CA) according to the manufacturers protocol. Barcoded libraries were pooled and sequenced using 125bp paired-end reads on the Illumina HiSeq 2500 system (Illumina, San Diego, CA). Bulk RNA-seq data for the nasal and tracheal ALI cultures to measure ACE2 and TMPRSS2 levels reported in Figures 3b ,c and 4g ,h , was generated with KAPA Hyperprep Stranded mRNA-seq library kits (Roche Sequencing and Life Science, Kapa Biosystems, Wilmington, MA) and sequenced with a Novaseq 6000 using 150bp paired end reads.
Biomek fxp automation system
Manufactured by Beckman Coulter
The Biomek FXP automation system is a liquid handling platform designed for a variety of laboratory applications. It features advanced pipetting capabilities, flexible deck configurations, and intuitive software to automate various liquid handling tasks.
Automatically generated - may contain errors
2 protocols using biomek fxp automation system
1
Transcriptome analysis of nasal airway epithelial cells
2
Nasal Airway Epithelial RNA Extraction
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Total RNA was extracted from the nasal airway epithelial brushings of GALA II subjects (n = 695) using the AllPrep DNA/RNA Mini Kit (QIAGEN, Germantown, MD). We used 250 ng of total RNA as input for the KAPA Stranded mRNA-seq library kit (Roche Sequencing and Life Science, Kapa Biosystems, Wilmington, MA), using the Beckman Coulter Biomek FXP automation system (Beckman Coulter, Fullerton, CA). Barcoded libraries were pooled and sequenced on the Illumina HiSeq 2500 system using 125 bp paired-end reads (Illumina, San Diego, CA).
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