Vapro 5600 vapor pressure osmometer

Manufactured by Wescor

Sourced in United States

The Vapro 5600 is a vapor pressure osmometer designed to measure the osmolality of a sample. It uses the freezing point depression method to determine the osmotic concentration of a liquid sample.

Automatically generated - may contain errors

Lab products found in correlation

Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Chemically defined lipid concentrate, by Thermo Fisher Scientific (1 mentions) Sevencompact ph meter, by Mettler Toledo (1 mentions) S2770, by Merck Group (1 mentions) Eclipse 50i, by Nikon (1 mentions)

Spelling variants of this product

Vapor pressure osmometer (38 citations) Vapro 5600 (23 citations) VAPRO Vapor Pressure Osmometer (10 citations) VAPRO (8 citations) Vapro osmometer (6 citations) Vapro pressure osmometer (4 citations)

5 protocols using vapro 5600 vapor pressure osmometer

Measuring Preferential Solute Interactions

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Osmolalities of solutions of protein model compounds and/or KCl were measured on Wescor Vapro 5600 Vapor Pressure Osmometers (VPO) calibrated as previously described.^{40 (link),53 (link),94 (link)} Differences in osmolality (ΔOsm(m₂,m₃)) between a three-component solution (Osm(m₂,m₃)) and the corresponding two-component solutions (Osm (m₂), Osm(m₃)) were calculated using Eq. (1):

Δ O s m (m_{2}, m_{3})) = O s m (m_{2}, m_{3}) - (O s m (m_{2}) + O s m (m_{3})) .

These ΔOsm(m₂,m₃) quantify the free energy consequences of interactions between the two solutes in water, as shown in Eq. (2):

Δ O s m (m_{2}, m_{3}) = \frac{μ_{23}}{R T} m_{2} m_{3}

where μ₂₃ is the chemical potential partial derivative (∂μ₂/∂m₃)_T.P,m2 which quantifies the preferential interaction of solute 3 (salt) with solute 2 (model compound), relative to interactions with water.^{95 –97 (link)} Hence the slope of a plot of ΔOsm(m₂,m₃) vs. m₂m₃ is μ₂₃/RT. In Eq. (2), the product m₂m₃ is the probability of an interaction of species 2 and 3, and

\frac{μ_{23}}{R T}

is the intrinsic strength of that interaction.

Kozlov A.G., Cheng X., Zhang H., Shinn M.K., Weiland E., Nguyen B., Shkel I.A., Zytkiewicz E., Finkelstein I.J., Record MT J.r, & Lohman T.M. (2022). How Glutamate Promotes Liquid-liquid Phase Separation and DNA Binding Cooperativity of E. coli SSB Protein. Journal of molecular biology, 434(9), 167562.

+ Open protocol

+ Expand

Quantifying Solute Interactions in Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Osmolalities of solutions of protein model compounds and/or KCl were measured on Wescor Vapro 5600 Vapor Pressure Osmometers (VPO) calibrated as previously described (Cheng et al., 2016 , Knowles et al., 2015 , Cheng et al., 2017) . Differences in osmolality (ΔOsm(𝑚2,𝑚3)) between a three-component solution (𝑂𝑠𝑚(𝑚2,𝑚3)) and the corresponding two-component solutions (𝑂𝑠𝑚(𝑚2), 𝑂𝑠𝑚(𝑚3)) were calculated using Eq. 1:
These ΔOsm(𝑚2,𝑚3) quantify the free energy consequences of interactions between the two solutes in water, as shown in Eq. 2:
where μ23 is the chemical potential partial derivative (𝜕μ2/𝜕m3)T.P,m2 which quantifies the preferential interaction of solute 3 (salt) with solute 2 (model compound), relative to interactions with water (Robinson and Stokes, 1961 , Capp et al., 2009 , Cheng et al., 2020) .
Hence the slope of a plot of Δ𝑂𝑠(𝑚2,𝑚3) vs. 𝑚2𝑚3 is μ23/RT. In Eq. 2, the product 𝑚 -𝑚 / is the probability of an interaction of species 2 and 3, and
56 is the intrinsic strength of that interaction.

Kozlov A.G., Cheng X., Zhang H., Shinn M.K., Weiland E., Nguyen B., Shkel I.A., Zytkiewicz E., Finkelstein I.J., Record M.T., & Lohman T.M. (2022). How Glutamate Promotes Liquid-liquid Phase Separation and DNA Binding Cooperativity ofE. coliSSB Protein.

+ Open protocol

+ Expand

Plant Growth and Osmotic Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols

The height of the main stem, the number of leaves on the main stem, the number of lateral branches, and the number of inflorescences on the main stem were measured every two weeks along with the experiment on 5 plants per accession and condition. The growth of the aerial part was estimated at the end of the experiment, based on the fresh (FW) and dry (DW) weights of the stems and leaves of 3 individuals per accession and condition. The dry weight was determined after 72 h of incubation in an oven at 70 °C. The leaf and stem water content (WC) was calculated as (FW − DW)/FW × 100.
For the determination of osmotic potential (Ψs), leaves from 3 plants per accession and condition were collected, cut into small fragments, and placed in pierced Eppendorf tubes. The samples were then subjected to 3 freeze-thaw cycles; each tube was then inserted into a second Eppendorf tube and then centrifuged at 9000× g for 10 min at 4 °C. The osmolarity in the extracted sap was analyzed with an osmometer (Wescor vapro 5600 vapor pressure osmometer, Logan, UT, USA) and converted into Ψs according to the Van’t Hoff equation [55 (link)].

Blanchard-Gros R., Bigot S., Martinez J.P., Lutts S., Guerriero G, & Quinet M. (2021). Comparison of Drought and Heat Resistance Strategies among Six Populations of Solanum chilense and Two Cultivars of Solanum lycopersicum. Plants, 10(8), 1720.

+ Open protocol

+ Expand

Optimizing Pluripotent Cell Culture

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All pluripotent and reprogramming cell cultures were maintained at 37°C with 5% CO₂ and 5% O₂. Differentiation cultures were maintained at 5% CO₂ and atmospheric (∼21%) O₂. E8 medium was made in house as previously described (Burridge et al., 2015 , Chen et al., 2011 (link)). Other medium components tested were human Long R3 IGF1 (Sigma, 91590C), thiazovivin (LC Labs, T-9753), recombinant human TGF-β3 (Cell Guidance Systems, GFH109), sodium bicarbonate (Sigma, S5761), NEAA (Gibco, 11140050), Chemically Defined Lipid Concentrate (Gibco, 11905031), fatty acid-free BSA (GenDEPOT, A0100), and heparin sodium salt (Sigma H3149-250KU). The pH was adjusted with 1 N HCl (Sigma, H9892) or 1 N NaOH (Sigma, S2770) and measured at room temperature and atmospheric CO₂ using a SevenCompact pH meter (Mettler Toledo). Osmolarity was adjusted with sodium chloride (Sigma, S5886) or cell culture water (Corning, 25-055-CV) and measured with a Vapro 5600 vapor pressure osmometer (Wescor).

Kuo H.H., Gao X., DeKeyser J.M., Fetterman K.A., Pinheiro E.A., Weddle C.J., Fonoudi H., Orman M.V., Romero-Tejeda M., Jouni M., Blancard M., Magdy T., Epting C.L., George AL J.r, & Burridge P.W. (2020). Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture. Stem Cell Reports, 14(2), 256-270.

+ Open protocol

+ Expand

Fungal Spore Germination Inhibition by Sweet Potato Extract

Check if the same lab product or an alternative is used in the 5 most similar protocols

Preparation, extraction and purification of sweet potato (Ipomoea batatas L. cv. Georgia Jet) active fraction (SPAF) were performed according to Turgeman et al. [7 (link)]. Fungal spores were treated with SPAF in purified water as the test treatment or purified water as a negative control. To examine the effect of pH on R. delemar spore germination, 20 mg/ml SPAF solution was adjusted for pH with 0.1 M HCl or 1 M NaOH and the level of osmolarity was 40+/-2 mOsm for pH 2.5–8.5 (using a Vapro 5600 vapor pressure osmometer, Wescor, Logan, UT). Spore solution (0.5 ml) at a final concentration of 5 x 10⁵ spore/ml was transferred into 50-ml tubes containing 0.5 ml SPAF and 4 ml purified water, or 4.5 ml purified water. The treated spores were then incubated in an orbital shaker at 30°C and 100 rpm. Spore germination was examined after 3 h and 24 h by mixing the solution and placing a 10-μl drop (in duplicate) onto a glass slide (Diagnostic microscope slides, Marienfeld-Superior, Lauda, Germany) and observing it under a light microscope (Eclipse 50i, Nikon, Japan). For photography, 1 ml of the sample at each studied time point was spun down (4°C, 2500 x g) and the spores were fixed with 3.7% (v/v) formaldehyde to prevent further growth. Pictures were taken at the same magnification (X100) with a digital camera (DS-Fi1 Nikon, Japan) mounted on the microscope.

Turgeman T., Shatil-Cohen A., Moshelion M., Teper-Bamnolker P., Skory C.D., Lichter A, & Eshel D. (2016). The Role of Aquaporins in pH-Dependent Germination of Rhizopus delemar Spores. PLoS ONE, 11(3), e0150543.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!