Mouse anti gst

The cells and tissues were lysed with an appropriate amount of RIPA lysis solution (Applygen, China) containing protease inhibitors, PMSF, and β-ME. The proteins were separated by SDS-PAGE and then transferred to PVDF membranes for primary antibodies incubation and secondary antibodies incubation. Mouse anti-GFP and mouse anti-β-actin were purchased from Abbkine. Rabbit anti-calcineurin A, mouse anti-GST, and rabbit anti-NFAT1 were purchased from Cell Signalling Technology. Rabbit anti-lamin B1 was provided by Proteintech. Goat anti-mouse IgG and goat anti-rabbit IgG secondary antibodies were purchased from SGB-BIO. After the secondary antibody incubation was completed, ECL luminescent solution (Tanon, China) was used for colour development. A Tanon instrument was used to obtain images, and ImageJ was used for image analysis.