HeLa cells were maintained in growth medium composed of DMEM (Sigma-Aldrich; D5648) supplemented with 3.7 g/l sodium bicarbonate (Sigma-Aldrich; S5761) plus 10% fetal bovine serum (Life Technologies; 10270-106) and 100 U/ml penicillin and streptomycin (Life Technologies; 15140-122) at 5% CO2 and 37°C. Autophagy was induced by washing cells with DPBS (Sigma-Aldrich; D5773) and incubating them in starvation media/Earle’s balanced salt solution (Sigma-Aldrich; E7510) made to 1× and supplemented with 2.2 g/l sodium bicarbonate for 2 h until otherwise stated.
D5773
Manufactured by Merck Group
Sourced in United States
D5773 is a laboratory equipment product offered by Merck Group. It is designed for general laboratory use, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. More information may be available from the manufacturer.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
D5648, by Merck Group (1 mentions)
E7510, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Vk x200, by Keyence (1 mentions)
2 protocols using d5773
1
Autophagic Induction in HeLa Cells
2
Characterizing Membrane Surface Topography
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Laser scanning microscopy was performed in all membrane formulations after immersion in phosphate-buffered saline (PBS, product reference D5773, Sigma–Aldrich, St. Louis, MO, USA) solution and incubation for 1 h at 35 °C. Topographic images and roughness measurements were taken on both sides of the membranes by scanning the Z-axis in high resolution with a laser scanning confocal microscope (Keyence, Itasca, IL, USA, model VK-X200), using a wavelength of 408 nm.
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