Anti tom20 sc 17764

Primary antibodies anti-EEA1 (ab70521), anti-58K Golgi (ab27043), antipericentrin (ab28144), and anticatalase (ab110292) were purchased from Abcam, anti-TGN46-8 (SAB4200355) from Sigma and anti-Tom20 (sc-17764) from Santa Cruz Biotechnology. All primary antibodies were monoclonal mouse antibodies. Polyclonal secondary antimouse antibodies conjugated to Atto647N (50185, Sigma Aldrich) as well as isotype-specific secondary antimouse antibodies conjugated to Atto647N (610-156-041, Rockland Immunochemicals) and Alexa Fluor 568 (A-21124, Thermo Fisher Scientific) were used for detection. The nanoboosters—single-domain alpaca antibody fragments covalently coupled to fluorescent dyes—GFP-Booster Atto488 and RFP-Booster Atto594 (Chromotek) were used to boost eYFP and mCherry fluorescence, respectively. Live cell stains SiR-tubulin, SiR-actin, and SiR-lysosome were from Spirochrome and MitoTracker Deep Red FM was from Thermo Fisher Scientific.

The antibodies used in this study were as follows: anti‐OGG1 (NB100‐106) and anti‐PINK1 (8E10.1D6) from Novus (Novus Biologicals), anti‐Parkin (sc‐32282) and anti‐TOM20 (sc‐17764) from Santa Cruz, anti‐LC3 (12741), and anti‐BCL2 (3498 S) from CST, anti‐COXIV (ab202554) from Abcam, anti‐α‐tubulin (11224‐1‐AP), anti‐BAX (60267‐1‐Ig), and anti‐cyclophilin B (11607‐1‐AP) from ProteinTech. All the secondary antibodies were purchased from Thermo Fisher Scientific. The specific inhibitor, Th5487, of OGG1 (HY‐125276) was purchased from Med Chem Express.