Human whole blood was collected from healthy donors (female, Caucasian) through venepuncture using K3EDTA vacuettes (Greiner bio-one, Kremsmünster, Austria). Eosinophils were directly isolated with the EasySepTM direct human eosinophil isolation Kit (StemCell, Cologne, Germany) as per the manufacturer’s description, and diluted to a final concentration of 1 × 106 cells per mL in 1xPBS 1 mM EDTA.
K3edta vacuettes
Manufactured by Greiner
Sourced in Austria
K3EDTA vacuettes are laboratory blood collection tubes used to collect venous blood samples. They contain the anticoagulant tripotassium ethylenediaminetetraacetic acid (K3EDTA) which prevents the blood from clotting.
Automatically generated - may contain errors
4 protocols using k3edta vacuettes
1
Direct Isolation of Human Eosinophils
2
Isolation of Human Eosinophils
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The blood was collected from healthy female Caucasian donors by means of venipuncture using K3EDTA vacuettes (Greiner bio-one, Kremsmünster, Austria). According to the manufacturer’s description, human eosinophils were isolated using EasySepTM direct eosinophil isolation kit (StemCell, Technologies, Vancouver, BC, Canada) and diluted to a final concentration of 1 × 106 cells per mL in 1x PBS 1 mM EDTA.
3
Comprehensive Biospecimen Analysis Protocol
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The investigated sample cohort was collected at the Department of Internal Medicine and Hematology, the 1 Department of Pathology and Experimental Cancer Research, Semmelweis University, and the Hungarian University of Sports Science, Budapest, Hungary. In this study, the following samples were involved: 180 fresh frozen (FF) and 25 formalin-fixed paraffin-embedded tissue (FFPE) biopsies, along with 38 and 12 blood specimens taken into standard K3EDTA Vacuettes (Greiner Bio-One Gmbh) and Cell-Free DNA Collection Tubes (Roche), respectively. The research has been approved by the local ethics committee (Regional and Institutional Committee of Science and Research Ethics; TUKEB Nr: 14383-2/2017/EKU). Written informed consent was obtained from all the involved patients prior to specimen collection.
4
Monocyte Isolation from Whole Blood
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Human whole blood was obtained from healthy female volunteers by venipuncture in K3EDTA vacuettes (Greiner bio-one, Kremsmünster, Austria). Following dilution of whole blood in HBSS without calcium and magnesium (ThermoFisher, Waltham, MA, USA), cells were layered over Ficoll Paque Plus (Cytiva, Marlborough, MA, USA) and separated using density centrifugation. Following the removal of the plasma layer, the buffy coat was aspirated and washed thrice with Hank’s balanced salt solution without magnesium and calcium. Cells were counted, and monocytes were assumed to be 1/6 of the total PBMC cell content.
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