Verikine hs mouse ifn beta elisa kit

Manufactured by PBL Assay Science

The VeriKine-HS Mouse IFN Beta ELISA Kit is a laboratory tool designed to detect and quantify mouse interferon beta (IFN-β) in biological samples using an enzyme-linked immunosorbent assay (ELISA) format. The kit provides the necessary reagents and protocols to perform this measurement.

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Lab products found in correlation

M csf, by Thermo Fisher Scientific (1 mentions) Murine tnf α, by Thermo Fisher Scientific (1 mentions) Control rabbit igg, by Santa Cruz Biotechnology (1 mentions) Recombinant mouse ifn β, by PBL Assay Science (1 mentions) Mojosort mouse cd45 nanobeads, by BioLegend (1 mentions) Mouse ifn alpha all subtype elisa kit, by PBL Assay Science (1 mentions) Softmax pro 3, by Molecular Devices (1 mentions) P nitrophenyl phosphate substrate, by Merck Group (1 mentions)

Spelling variants of this product

Verikine Mouse IFN Beta ELISA Kit (7 citations) Mouse IFN Beta ELISA Kit (7 citations) VeriKine-HS Mouse IFN Beta Serum ELISA Kit (2 citations)

2 protocols using verikine hs mouse ifn beta elisa kit

Murine Cytokine and IFN-beta Assays

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Murine TNF-α and M-CSF were purchased from Peprotech. Recombinant mouse IFN-β was from PBL Assay Science. The control IgG (rabbit) was obtained from Santa Cruz Biotechnology, and IFN-β neutralizing antibody (rabbit polyclonal antibody against mouse interferon-beta) was from PBL Assay Science. Mouse IFN Alpha All Subtype ELISA Kit and VeriKine-HS Mouse IFN Beta ELISA Kit were purchased from PBL Assay Science. MojoSort Mouse CD45 Nanobeads were obtained from Biolegend.

Deng Z., Ng C., Inoue K., Chen Z., Xia Y., Hu X., Greenblatt M., Pernis A, & Zhao B. (2020). Def6 regulates endogenous type-I interferon responses in osteoblasts and suppresses osteogenesis. eLife, 9, e59659.

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Quantification of Anti-Chromatin and Anti-dsDNA

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Anti-chromatin and anti-dsDNA were measured by ELISA using sonicated chicken chromatin and calf thymus dsDNA, respectively. Serum samples were tested at a 1:250 dilution followed by incubation with alkaline phosphatase-labeled goat anti-mouse IgG (1:1000 dilution) (Southern Biotechnology), and development with p-nitrophenylphosphate substrate (Sigma-Aldrich). Optical density at 405 nm was read using a VERSAmax microplate reader (Softmax Pro 3.1 software; Molecular Devices). Sera IFN-β concentrations were measured using the VeriKine-HS Mouse IFN Beta ELISA kit from PBL assay science following manufacture’s protocol.

Dong X., Antao O.Q., Song W., Sanchez G.M., Zembrzuski K., Koumpouras F., Lemenze A., Craft J, & Weinstein J.S. (2021). Type 1 interferon activated STAT4 regulates T follicular helper (Tfh)-cell dependent cytokine and immunoglobulin production in lupus. Arthritis & rheumatology (Hoboken, N.J.), 73(3), 478-489.

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