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Mouse anti biotin

Manufactured by Santa Cruz Biotechnology

The Mouse anti-Biotin antibody is a monoclonal antibody that specifically binds to the small molecule biotin. This antibody is commonly used in various laboratory techniques, such as Western blotting, ELISA, and immunohistochemistry, to detect and localize biotin-labeled molecules.

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2 protocols using mouse anti biotin

1

Immunoblotting Antibody Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysates were analysed by standard chemiluminescent immunoblotting techniques. Primary antibodies: mouse anti-actin 1:2000 (C-4; Sigma Aldrich), mouse anti-Biotin 1:400 (33; Santa Cruz Biotechnology), rat anti-Ft 1:500 (Sopko et al., 2009 (link)), mouse anti-FLAG 1:5000 (M2; Sigma Aldrich), mouse anti-GST 1:500 (B-14; Santa Cruz Biotechnology), rabbit anti-HA 1:1000 (C29F4; Cell Signaling Technology), rat anti-HA 1:2000 (3F10; Roche Applied Science) and mouse anti-V5 1:5000 (R960-25; Thermo Fisher Scientific).
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2

Immunoblotting Antibody Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysates were analysed by standard chemiluminescent immunoblotting techniques. Primary antibodies: mouse anti-actin 1:2000 (C-4; Sigma Aldrich), mouse anti-Biotin 1:400 (33; Santa Cruz Biotechnology), rat anti-Ft 1:500 (Sopko et al., 2009 (link)), mouse anti-FLAG 1:5000 (M2; Sigma Aldrich), mouse anti-GST 1:500 (B-14; Santa Cruz Biotechnology), rabbit anti-HA 1:1000 (C29F4; Cell Signaling Technology), rat anti-HA 1:2000 (3F10; Roche Applied Science) and mouse anti-V5 1:5000 (R960-25; Thermo Fisher Scientific).
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