PSB and BALF samples were inoculated into Eosin methylene blue agar, Brucella agar, blood agar, and chocolate agar media (Biomérieux, Marcy l'Etoile. France); and incubated for 24–48 h at 37 °C. Colony identification was performed using the Vitek 2 Compact full automatic identification system (Biomérieux, France). For PSB and BALF cultures, after 24 h of incubation, bacterial colonies with growth of ≥103 and ≥105, respectively, were considered as pathogenic.
Eosin methylene blue agar
Manufactured by bioMérieux
Sourced in France
Eosin methylene blue agar is a selective and differential culture medium used for the isolation and differentiation of Gram-negative bacteria, particularly Escherichia coli and other coliforms. It contains eosin and methylene blue as dyes, which inhibit the growth of Gram-positive bacteria and allow the differentiation of Gram-negative colonies based on their lactose fermentation ability and subsequent color change.
Automatically generated - may contain errors
2 protocols using eosin methylene blue agar
1
Bacterial Culture and Identification
2
Urinary Tract Infection Surveillance Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In this retrospective study, all urinary samples from both outpatients and inpatients
sent to the microbiology laboratory of Ondokuz Mayıs University Medical Center from
January 2012 to December 2020 were enrolled. In addition, demographic data, such as
patient age and gender, and laboratory data were extracted from the hospital
records. Only one urinary culture from each patient was included in the study.
Urine samples were routinely inoculated onto 5% sheep blood agar (bioMérieux, Marcy
l'Etoile, France) and eosin methylene blue agar (bioMérieux, Marcy l'Etoile, France)
and the plates were then incubated for 20-22 hours at 35 oC. The VITEK® MS automated system (bioMérieux, Marcy l'Etoile, France) was used for
identification. The antimicrobial susceptibility and ESBL production of the isolates
was determined using the VITEK® 2 Compact (bioMérieux, Marcy l'Etoile,
France) and evaluated according to the European Committee on Antimicrobial
Susceptibility Testing (EUCAST) Guideline specific to that year.
The study was approved by Ondokuz Mayıs University Medical Faculty Ethics Committee
(B.30.2.ODM.0.20.08/498).
Statistical Analysis The association between years and fosfomycin resistance rates was analyzed with the
ANOVA test, and the association between outpatients/inpatients and fosfomycin
resistance rates was analyzed with Pearson's χ2 test. The statistical significance
was set as p<0.05.
sent to the microbiology laboratory of Ondokuz Mayıs University Medical Center from
January 2012 to December 2020 were enrolled. In addition, demographic data, such as
patient age and gender, and laboratory data were extracted from the hospital
records. Only one urinary culture from each patient was included in the study.
Urine samples were routinely inoculated onto 5% sheep blood agar (bioMérieux, Marcy
l'Etoile, France) and eosin methylene blue agar (bioMérieux, Marcy l'Etoile, France)
and the plates were then incubated for 20-22 hours at 35 oC. The VITEK® MS automated system (bioMérieux, Marcy l'Etoile, France) was used for
identification. The antimicrobial susceptibility and ESBL production of the isolates
was determined using the VITEK® 2 Compact (bioMérieux, Marcy l'Etoile,
France) and evaluated according to the European Committee on Antimicrobial
Susceptibility Testing (EUCAST) Guideline specific to that year.
The study was approved by Ondokuz Mayıs University Medical Faculty Ethics Committee
(B.30.2.ODM.0.20.08/498).
ANOVA test, and the association between outpatients/inpatients and fosfomycin
resistance rates was analyzed with Pearson's χ2 test. The statistical significance
was set as p<0.05.
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