Pe anti human cd62l

We detected the expression of CD40 in the different subsets of T cells before or after stimulation for different times. The antibodies used included BB515 mouse anti-human CD40 (BD Horizon), APC-Cy7 mouse anti-human CD8 (BD Pharmingen), PerCP-Cy5.5 Mouse anti-human CD4 (BD Pharmingen) and BV510 mouse anti-human CD3 (BD Horizon) antibodies were used. On the 10th day after electroporation, CAR was expressed on the surface of the cells, and the cells were stained with biotin-conjugated rhMSLN antigen (ACROBiosystems) and PE-conjugated streptavidin (BD Biosciences). The following monoclonal antibodies were used for the phenotypic analysis: PE-Cy5 anti-human CD45RO (BioLegend), PE anti-human CD62L (BioLegend), FITC anti-human CD197 (BioLegend), PE-Cy5 anti-human CD25 (BioLegend), PC5 anti-human CD69 (BioLegend), and PE-CY5 anti-human CD107α (BD Biosciences). The surface expression of MSLN on SKOV-3 cells was detected using a primary self-synthesized anti-MSLN antibody with Fc fragments and a PE anti-human IgG Fc secondary antibody (BioLegend). The acquisition and analysis were performed using a Navios™ flow cytometer (Beckman Coulter, USA) and Kaluza Analyse software.