A total of 43 male mice were used in this study and the figure contribution of each mouse is summarized in Supplemental table 1 . All mice tested were between 2 and 12 month of age. C57Bl/6J (WT) mice were purchased from Taconic Biosciences and VGAT-ChR2 mice were obtained from the Jackson laboratories. VGAT-cre mice were backcrossed to C57Bl/6J mice for at least six generations. All mice were kept on a 12 h light-dark cycle. All animal experiments were performed according to the guidelines of the US National Institutes of Health and the Institutional Animal Care and Use Committee at the New York University Langone Medical Center.
Vgat chr2 mice
Manufactured by Jackson ImmunoResearch
VGAT-ChR2 mice express the light-sensitive cation channel channelrhodopsin-2 (ChR2) under the control of the vesicular GABA transporter (VGAT) promoter, which drives expression in GABAergic neurons. This allows for the optical control of GABAergic neuronal activity.
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4 protocols using vgat chr2 mice
1
Behavioral Experiments on C57Bl/6J and VGAT-ChR2 Mice
2
Optogenetic Control of GABAergic Neurons in Mice
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All animal procedures were approved by the Institutional Animal Care and Use Committee of The Jikei University (no. 2015-054 and 2017-001) under the Guidelines for the Proper Conduct of Animal Experiments of the Science Council of Japan (2006). Hemizygous transgenic mice (3–8 weeks old, male and female) expressing modified ChR2 (hChR2-H134R) fused with YFP in GABAergic neurons via the vesicular GABA transporter promoter/enhancer (VGAT-ChR2 mice, stock no. 014548; Jackson Laboratory)69 (link) were used in photoinhibition experiments. Heterozygous knock-in mice (3–5 weeks old, male and female) expressing Venus fluorescent protein via the aldolase C promoter (Aldoc-Venus mice, MGI:3609644)70 (link) were used to visually identify the cerebellar parasagittal zones.
3
Behavioral Experiments on C57Bl/6J and VGAT-ChR2 Mice
4
VGAT-ChR2 Mice for Neuroscience
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VGAT-ChR2 mice were obtained from the Jackson Labs and maintained on a C57Bl6/J background. VGAT-Cre mice were backcrossed to C57Bl6/J mice for at least six generations. All experiments were conducted according to the guidelines of the Institutional Animal Care and Use Committee (IACUC) at Massachusetts Institute of Technology (MIT), the New York University Langone Medical Center and the US National Institutes of Health.
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