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Il 4 fitc

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IL-4 FITC is a fluorescent-labeled antibody used in flow cytometry applications to detect and quantify interleukin-4 (IL-4) expression in biological samples. IL-4 is a cytokine that plays a crucial role in the regulation of immune responses.

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Lab products found in correlation

Cd45ra fitc, by BioLegend (3 mentions) Cd4 pe, by BD (2 mentions) Foxp3 fitc, by Thermo Fisher Scientific (2 mentions) Cd80 fitc, by BD (2 mentions) Cd4 fitc, by BD (2 mentions) Cd86 fitc, by BD (2 mentions) Cd25 pe, by BioLegend (2 mentions) Cd4 pc7, by BioLegend (2 mentions) Human ifn γ capture and detection antibodies, by Mabtech (2 mentions) Cd4 pc5, by BioLegend (2 mentions) Il 10 pe, by BioLegend (2 mentions) Cd3 percp cy5, by BD (1 mentions) Cd4 apc, by BD (1 mentions) Foxp3 pe, by Thermo Fisher Scientific (1 mentions) Cd80 fitc, by Thermo Fisher Scientific (1 mentions) Hla dr apc, by BD (1 mentions) Mp4 25d2, by BioLegend (1 mentions) Cd27 pe cy7, by Thermo Fisher Scientific (1 mentions) Btla pe, by BD (1 mentions) Cd11c pe cy7 b ly6, by BD (1 mentions)

3 protocols using il 4 fitc

1

Flow Cytometry and Cell Sorting

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The following mouse anti-human antibodies were used for flow cytometry and cell sorting: CD11c-APC-eFluor780 (BU15, eBioscience), CD11c-PE-Cy7 (B-ly6, BD Pharmingen), Lin1-FITC (SK7, 3G8, SJ25C1, L27, eMfP9, NCAM16.2, BD Biosciences), Lin1-APC (UCHT1, HCD14, 3G8, HIB19, 2H7, HCD56, BioLegend), CD123-PerCP-CyTM5.5 (7G3, BD Biosciences), HLA-DR-APC-eFluor780 (LN3, eBioscience), HLA-DR-APC (G46-6, BD), CD86-FITC (FUN-1, BD Biosciences), CD80-FITC (2D10.4, eBioscience), CD83-FITC (HB15e, BD Biosciences), CCR7-FITC (150503, BD Biosciences), BTLA-APC (MIH26, Biolegend), BTLA-PE (J168-540, BD Pharmingen), CD3-PerCp-Cy5.5 (SP34-2, BD Biosciences), CD4-PerCP-Cy5.5 (RPA-T4, eBioscience), CD4-APC (L200, BD Biosciences), CD27-PE-Cy7 (0323, eBioscience), CD45RA-FITC (HI100, Biolegend), CD27-PE-Cy7 (0323, eBioscience), CD45RA-FITC (HI100, Biolegend), Foxp3-PE (PCH101, eBioscience), IFN-α-FITC (MMHA-11, InterferonSource), IL-12-FITC (MHCIL1201, Invitrogen), IL-4-FITC (MP4-25D2, Biolegend), and IFN-γ-APC-eFluor780 (45.B3, eBioscience).
Zhang J.A., Lu Y.B., Wang W.D., Liu G.B., Chen C., Shen L., Luo H.L., Xu H., Peng Y., Luo H., Huang G.X., Wu D.D., Zheng B.Y., Yi L.L., Chen Z.W, & Xu J.F. (2020). BTLA-Expressing Dendritic Cells in Patients With Tuberculosis Exhibit Reduced Production of IL-12/IFN-α and Increased Production of IL-4 and TGF-β, Favoring Th2 and Foxp3+ Treg Polarization. Frontiers in Immunology, 11, 518.
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2

Multiparametric Flow Cytometry Assay

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The following antibodies were purchased from BD Biosciences: CD4 FITC, CD4 PE, HLA-ABC FITC (MHC I), HLA-DP,DQ,DR FITC (MHC II), CD80 FITC, and CD86 FITC. The following antibodies were purchased from Biolegend (San Diego, CA): CD4 PC5, CD4 PC7, CD45RA FITC, IFN-γ PC7, IL-10 PE, IL-4 FITC, and CD25 PE. The following antibody was purchased from eBioscience (San Diego, CA): Foxp3 FITC. Appropriate isotype controls were purchased from either BD Biosciences or Biolegend. Human IFN-γ capture and detection antibodies were purchased from Mabtech (Cincinnati, OH). Poly-ICLC (Hiltonol) is a clinical grade current good manufacturing practices (cGMP) poly-lysine stabilized form of Poly polyinosinic-polycytidylic acid (PolyIC) provided by Oncovir, Inc. (Washington, D.C.).
Woodham A.W., Yan L., Skeate J.G., van der Veen D., Brand H.E., Wong M.K., Da Silva D.M, & Kast W.M. (2016). T cell ignorance is bliss: T cells are not tolerized by Langerhans cells presenting human papillomavirus antigens in the absence of costimulation. Papillomavirus Research, 2, 21-30.
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3

Multiparameter Flow Cytometry Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were purchased from BD Biosciences: CD4 FITC, CD4 PE, HLA-ABC FITC (MHC I), HLA-DP,DQ,DR FITC (MHC II), CD80 FITC, and CD86 FITC. The following antibodies were purchased from Biolegend (San Diego, CA): CD4 PC5, CD4 PC7, CD45RA FITC, IFN- PC7, IL-10 PE, IL-4 FITC, and CD25 PE. The following antibody was purchased from eBioscience (San Diego, CA): FoxP3 FITC. Appropriate isotype controls were purchased from either BD Biosciences or Biolegend. Human IFN-γ capture and detection antibodies were purchased from Mabtech (Cincinnati, OH). Poly-ICLC (Hiltonol) is a clinical grade current good manufacturing practices (cGMP) poly-lysine stabilized form of Poly polyinosinic-polycytidylic acid (PolyIC) provided by Oncovir, Inc. (Washington, D.C.).
Woodham A.W., Yan L., Skeate J.G., van der Veen D., Brand H.E., Wong M.K., Da Silva D.M, & Kast W.M. (2016). T cell ignorance is bliss: T cells are not tolerized by Langerhans cells presenting human papillomavirus antigens in the absence of costimulation. Papillomavirus Research, 2, 21-30.
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