Western lightning chemiluminescence plus detection system

Manufactured by PerkinElmer

Sourced in United States

The Western Lightning Chemiluminescence Plus detection system is a laboratory equipment designed for the analysis of protein expression through Western blotting. It utilizes chemiluminescence technology to detect and quantify target proteins on a membrane.

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Lab products found in correlation

Antibody against actin, by Merck Group (1 mentions) Acetyl h4, by Merck Group (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) Bcl2 associated x (bax), by Santa Cruz Biotechnology (1 mentions) M per reagent, by Thermo Fisher Scientific (1 mentions) Bca reagent, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Western Lightning (69 citations) Western Lightning Plus (39 citations) Chemiluminescence detection system (16 citations) Chemiluminescence system (6 citations) Western Lightning Plus system (5 citations) Western Lightning System (5 citations) Western Lightning Chemiluminescence Reagent Plus detection system (2 citations)

2 protocols using western lightning chemiluminescence plus detection system

Protein Expression Analysis in Treated Cells

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Total protein was extracted from treated cells using the M-PER mammalian protein extraction reagent (Pierce). Protein concentration was determined using the BCA Reagent (Pierce). Protein extracts were analyzed using antibodies for the detection of phospho-GSK3β^Ser-9, GSK3β, β-catenin, PARP, Bcl-2 (Cell Signaling Technologies), Acetyl H4 (Millipore) and Bax (Santa Cruz). Antibody against actin (Sigma) was used to normalize protein loading in each lane. Bands were visualized using the Western Lightning Chemiluminescence Plus detection system (PerkinElmer), according to the manufacturer's protocol.

Thotala D., Karvas R.M., Engelbach J.A., Garbow J.R., Hallahan A.N., DeWees T.A., Laszlo A, & Hallahan D.E. (2015). Valproic acid enhances the efficacy of radiation therapy by protecting normal hippocampal neurons and sensitizing malignant glioblastoma cells. Oncotarget, 6(33), 35004-35022.

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Protein Quantification and Phosphorylation Analysis

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Cell lysates were prepared using M-PER reagent (Thermo Scientific, Waltham, MA, USA). Lysates were centrifuged at 17,000×g for 15 min at 4°C, and total protein concentration was determined using BCA reagent (Thermo Scientific). Protein extracts were subjected to Western immunoblot analysis and developed using the Western Lightning Chemiluminescence Plus detection system (PerkinElmer, Wellesley, MA, USA) according to the manufacturer’s protocol. Densitometry was performed using ImageJ program. To quantify levels of protein phosphorylation, OD of bands for phosphoprotein was normalized to total protein and β-actin.

Yazlovitskaya E.M., Viquez O., Tu T., De Arcangelis A., Georges-Labouesse E., Sonnenberg A., Pozzi A, & Zent R. (2018). The laminin binding α3 and α6 integrins cooperate to promote epithelial cell adhesion and growth. Matrix biology : journal of the International Society for Matrix Biology, 77, 101-116.

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