Cells were stained with PE-Cy7 anti-F4/80, PE-Cy7 anti-CD11c, PerCP anti-CD3, PE anti-PD-L1, APC anti-CD86, FITC-CD206, PE anti-Foxp3, APC anti-CD25, and APC anti-IFNγ (BioLegend, San Diego, CA). Labile cell iron was measured using 10 μM calcein acetoxymethyl (calcein_AM, Thermo Fisher Scientific, MA). Lipid peroxidation was assessed by C11-BODIPY 581/591 (Thermo Fisher Scientific, MA), and the amount of ROS was measured by a DCFDA cellular ROS detection assay kit (Abcam, Cambridge, UK). CD4+IFNγ+ cells were detected by an intracellular staining kit (Fixation/Permeabilization Solution Kit, Franklin Lakes, NJ), and CD4+CD25+Foxp3+ cells were measured using a Foxp3/transcription factor staining buffer set (eBioscience). All data were analyzed using FACS LSR Fortessa cytometry with BD CELL Quest Pro software.
Facs lsr fortessa cytometry
Manufactured by BD
Sourced in United States
The FACS LSR Fortessa cytometry system is a high-performance flow cytometer designed for advanced multi-parameter analysis. It offers precise detection and quantification of fluorescent signals from cells or particles in suspension.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd86 apc, by BioLegend (2 mentions)
Anti cd11c pe cy7, by BioLegend (2 mentions)
Cellquest pro software, by BD (2 mentions)
Dcfda cellular ros detection assay kit, by Abcam (2 mentions)
Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions)
Anti ifn γ apc, by BioLegend (1 mentions)
Anti cd3 percp, by BioLegend (1 mentions)
Cd206 fitc, by BioLegend (1 mentions)
Anti pd l1 pe, by BioLegend (1 mentions)
Anti cd25 apc, by BioLegend (1 mentions)
Anti foxp3 pe, by BioLegend (1 mentions)
Fixation permeabilization solution kit, by Thermo Fisher Scientific (1 mentions)
Bodipy 581 591 c11, by Thermo Fisher Scientific (1 mentions)
Pe cy7 anti f4 80, by BioLegend (1 mentions)
Mitotracker green, by Thermo Fisher Scientific (1 mentions)
Anti cd80 pe, by BioLegend (1 mentions)
Mitotracker deep red, by Thermo Fisher Scientific (1 mentions)
2 protocols using facs lsr fortessa cytometry
1
Multiparametric Analysis of Immune Cell Phenotypes
2
Multiparametric Analysis of Dendritic Cells
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Dendritic cells were stained with PE-Cy7-anti-CD11c, BV421-anti-CD40, PE-anti-CD80, and APC-anti-CD86 (BioLegend, USA) in phosphate-buffered saline for 30 min at 4°C. Lipid droplets were stained with 10 µM BODIPY493/503, and mitochondria membrane potentials were estimated with 20 nM MitoTracker Deep Red and 20 nM MitoTracker Green (Thermo Fisher Scientific, USA) for 30 min at 37°C. ROS was assayed by a DCFDA Cellular ROS Detection Assay Kit (Abcam, UK). Data were analyzed using FACS LSR Fortessa cytometry with BD CellQuest Pro software (BD, USA).
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