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Cy5 anti murine antibody

Manufactured by Abcam
Sourced in United Kingdom

The Cy5 anti-murine antibody is a fluorescently labeled secondary antibody that specifically binds to mouse primary antibodies. It is designed for use in various immunoassay techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to detect and visualize mouse target proteins.

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2 protocols using cy5 anti murine antibody

1

Apoptosis Signaling Pathway Profiling

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The following primary antibodies were used: mouse anti-active caspase-8 (Cell Signaling Technology, Beverly, USA), rabbit anti FAS, and anti FADD antibodies (Santa Cruz Biotechnology), murine activating anti-CD95/FAS (50 ng/ml) from upstate signaling, rabbit anti-AIF IgG (Sigma-Aldrich), mouse anti-p53 antibody (Millipore), Rabbit anti-Flag (Thermo/Fisher Scientific), rabbit anti-nur77 IgG (Santa Cruz), mouse anti–β-actin (Abcam). The following secondary antibodies were used: Infrared dye 800cw goat, anti-rabbit antibody, Infrared dye 680cw goat, anti-mouse antibody (Licor), Rhodamine redX anti-rabbit antibody (Life Technologies), cy5 anti-murine antibody (Abcam).
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2

Antibody Validation Protocol

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The following primary antibodies were used: mouse anti-® actin (Abcam, UK). α/β-Tubulin Antibody (Cell signaling), Anti-cyclin B1 (Millipore, Germany), Anti-phospho-Ser/Thr-Pro MPM-2 Antibody (Millipore, Germany). The following secondary antibodies were used: Infrared dye 800cw goat, anti-rabbit antibody, Infrared dye 680cw goat, anti-mouse antibody (Licor, USA), Rhodamine redX antirabbit antibody (life technologies), cy5 anti-murine antibody (Abcam, UK).
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