Api id32 phenotyping kits

Clinical isolates of Elizabethkingia species, obtained between 2005 and 2020, were collected from 5 hospitals in Taiwan, namely, E-Da Hospital, Kaohsiung Medical University Hospital, E-Da Cancer Hospital, National Cheng Kung University Hospital, and Taichung Veterans General Hospital. These isolates had been routinely collected from patients in accordance with clinical requirements. All isolates were initially identified as Elizabethkingia species by clinical microbiology laboratories using API/ID32 phenotyping kits (bioMérieux, Marcy l’Etoile, France), the Phoenix 100 ID/AST automated microbiology system (Becton, Dickinson Co., Sparks, MD, USA), the Vitek 2 automated identification system (bioMérieux), or the Vitek MALDI-TOF MS system (bioMérieux). Isolates were stored as glycerol stocks at −80°C until use.

This study was approved by the Institutional Review Board of E-Da Hospital (EMRP-106-105) and was conducted in accordance with the Declaration of Helsinki and national and institutional standards. The computer database of the clinical microbial laboratory in a 1000-bed university-affiliated medical center in Taiwan was searched for cultures that yielded Elizabethkingia species between January 2005 and June 2018. The API/ID32 Phenotyping Kits (bioMérieux, Marcy l’Etoile, France) and VITEK MS System (bioMérieux, Marcy l’Etoile, France) were used for microbial identification by the clinical microbial laboratory during 2005–2013 and 2014–2018, respectively. All isolates were stored as glycerol stocks at −80 °C until use. The species of Elizabethkingia was reidentified using 16S rRNA gene sequencing. Patients infected with E. meningoseptica and E. anophelis were included in this study. Clinical characteristics and outcomes were collected from the medical records. Inappropriate empirical antimicrobial therapy was defined as nonsusceptibility of the isolates to the empirically prescribed antibiotics. Shock was defined as systolic pressure of <90 mm Hg, a reduction of 40 mm Hg in systolic blood pressure from baseline, or a condition requiring inotropic agents to maintain blood pressure.

Clinical isolates of Elizabethkingia species, obtained between 2005 and 2020, were collected from 5 hospitals in Taiwan, namely, E-Da Hospital, Kaohsiung Medical University Hospital, E-Da Cancer Hospital, National Cheng Kung University Hospital, and Taichung Veterans General Hospital. These isolates had been routinely collected from patients in accordance with clinical requirements. All isolates were initially identified as Elizabethkingia species by clinical microbiology laboratories using API/ID32 phenotyping kits (bioMérieux, Marcy l’Etoile, France), the Phoenix 100 ID/AST automated microbiology system (Becton, Dickinson Co., Sparks, MD, USA), the Vitek 2 automated identification system (bioMérieux), or the Vitek MALDI-TOF MS system (bioMérieux). Isolates were stored as glycerol stocks at −80°C until use.