Yeast extract powder

Hesperetin-7-O-glucoside, prunin, and isoquercitrin were prepared in our laboratory (Ye H. et al., 2022 (link); Hangzhou, China). Hesperidin, hesperetin, naringin, naringenin, rutin, and quercetin were obtained from Aladdin Reagent Co., Ltd. (Shanghai, China). Yeast extract powder, tryptone, crotonic acid, KH₂PO₄, K₂HPO₄, heme, MgSO₄, NaCl, CaCl₂, and L-cysteine were purchased from Sigma (Missouri, United States).

UCP (NaYF₄:Yb³⁺/Er³⁺), with a diameter of about 50 nm, was obtained from Shanghai Kerune Phosphor Technology Co. Ltd (Shanghai, China). The peaks of its excitation and emission spectra were 980 nm and 541.5 nm, respectively. Nitrocellulose membrane (SHF 1350225) and glass fibre (GFCP20300) were purchased from Millipore Corp. (Bedford, MA, USA). Papers (nos 470 and 903) used to make the sample pad and absorbent pad were obtained from Schleicher and Schuell, Inc. (Keene, NH, USA). The laminated cards and plastic cartridges of the TC-UPT-LF disc were designed by our group and manufactured by Shanghai Liangxin Biotechnology Co. (Shanghai, China) and Shenzhen Jincanhua Industry Co. (Shenzhen, China), respectively.
The reagents, including tryptone, yeast extract powder, NaCl, tris(hydroxymethyl)aminomethane, HCl, Na₂HPO₄, KH₂PO₄, and SDS were all of analytical grade and purchased from Sigma-Aldrich (St. Louis, MO, USA). Defatted milk powder was obtained from a local supermarket.

Larvae of Drosophila melanogaster were used as experimental organism to assess the toxicological impact of Acephate on hemocyte abundance. Larvae were reared in Standard Drosophila medium (SDM) following the established laboratory rearing techniques (after Dutta et al., 2014 ; and Podder et al., 2012 ). SDM ingredients included corn meal, sucrose (SRL India), agar agar (Merck, India) and yeast extract powder (Merck, India). Nepagin (Supelco, USA) and Propionic acid (Himedia, India) were added for their antifungal properties.