All oligonucleotides were obtained from Sangon Biotechnology Co., Ltd (Shanghai, China). All oligonucleotides and miRNAs were purified with HPLC. The sequence of oligos used is detailed in Table S1.† DEPC treated deionized water, 40% acrylamide, 6× loading buffer and 4S GelRed dye were purchased from Sangon Biotechnology Co., Ltd (Shanghai, China). Penicillin-streptomycin, 4-(2-hydroxyethyl) piperazine-1-ethylate sodium salt (HEPES), magnesium chloride, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazole bromide (MTT) were purchased from Sigma-Aldrich (MO, USA), and all were analytically pure reagents. Dulbecco's modified Eagle Culture medium (DMEM), fetal bovine serum (FBS) and trypsin were purchased from Thermo Fisher Scientific (USA). MCF-7 cells, MDA-MB-231 cells, A549 cells, MCF-10A cells and MRC-5 cells were purchased from Shanghai Institute for Biological Sciences (SIBS).
Gel electrophoresis was performed using a DyCZ-24DN electrophoresis cell (LIUYI, Beijing, China) and DigiGenius gel system (Syngene, UK). Fluorescence spectra were measured using a WATERS Prep 150 fluorescence spectrophotometer (WATERS, USA). Confocal fluorescence images of cells were obtained using a LSM 880 confocal laser scanning fluorescence microscope (Carl Zeiss, Germany). Cell viability was determined using a M1000PRO Multifunctional Microplate Reader (TECAN, Austria).
Gel electrophoresis was performed using a DyCZ-24DN electrophoresis cell (LIUYI, Beijing, China) and DigiGenius gel system (Syngene, UK). Fluorescence spectra were measured using a WATERS Prep 150 fluorescence spectrophotometer (WATERS, USA). Confocal fluorescence images of cells were obtained using a LSM 880 confocal laser scanning fluorescence microscope (Carl Zeiss, Germany). Cell viability was determined using a M1000PRO Multifunctional Microplate Reader (TECAN, Austria).