A total of 90 rats (200–250 g) survived the experiment and were randomly divided into two groups: a control group (ACNG group) and a test group (ACNG + COMP-Ang1 group). Each group consisted of 45 animals. All surgical procedures were performed by the same surgeon under aseptic operating conditions using 10% chloral hydrate anesthesia (0.3 mL/100 g body weight). The incision was made on the lateral side of the left thigh to expose the sciatic nerve. With the aid of a surgical microscope, a 10-mm segment of the nerve was removed and microsurgically repaired using a 12-mm ACNG. Penicillin G (10,000 U) (Sigma, Deisenhofen, Germany) was postoperatively injected into the quadriceps femoris for 3 days. In the ACNG + COMP-Ang1 group, all rats received daily intraperitoneal injections of COMP-Ang1 (5 μL/g body weight, Enzo Life Sciences, Farmingdale, NY, USA), while rats in the ACNG group received daily doses of a 0.9% NaCl solution (5 μL/g body weight, i.p.). All rats were housed in cages in a warm environment and permitted free access to food and water.
Comp ang1
Manufactured by Enzo Life Sciences
Sourced in United States
COMP-Ang1 is a recombinant protein product from Enzo Life Sciences. It is a truncated and stabilized form of the Angiopoietin-1 (Ang1) protein.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using comp ang1
1
Sciatic Nerve Repair in Rats
2
Molecular Mechanisms of Endothelial Barrier Regulation
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Unless otherwise stated, all reagents were purchased from Sigma-Aldrich (Dublin, Ireland). Western blotting primary antisera were purchased from the following sources: anti-occludin IgG, anti-claudin-5 IgG, and anti-ZO-1 IgG (Thermo Fischer Scientific, Dublin, Ireland); anti-VE-Cadherin IgG (Abcam, Cambridge, UK); anti-β-actin (Cell Signaling Technologies Inc., Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated secondary antisera for all targets were purchased from Cell Signaling Technologies Inc. COMP-Ang1 was purchased from Enzo Life Sciences (Exeter, UK). For Tie2 experiments, dual Ambion Silencer Select small interfering RNA (siRNA) constructs (assay IDs 13983 and s13984 against TEK/Tie2) were purchased from Thermo Fisher Scientific. An anti-Tie2 IgG and associated donkey anti-goat secondary IgG were purchased from R&D Systems (Abingdon, UK).
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