Derivatized lipids were separated with an Agilent 7890B Series GC equipped with 2 Agilent DB-17HT columns in tandem (each 30 m x 0.25 mm i.d. x 0.15 μm film thickness) with helium as the carrier gas at a constant flow of 1.1 ml/min. The program was as follows: 100 °C for 2 min, then 12 °C/min to 250 °C and held for 10 min, then 10 °C/min to 330 °C and held for 17.5 min. Two µL of each sample was injected in splitless mode at 250 °C. The GC was coupled to an Agilent 5977 A Series MSD with the ion source at 230 °C and operated at 70 eV in EI mode scanning from 50 to 850 Da in 0.5 s. Lipids were identified based on their retention time and compared to previously published spectra (Wei et al., 2016 (link)).
7890b series gc
Manufactured by Agilent Technologies
Sourced in United States
The 7890B Series GC is a gas chromatograph designed for analytical separation and detection of chemical compounds. It features a temperature-controlled oven, inlet systems, and detectors to facilitate the separation and analysis of complex samples. The 7890B is a versatile instrument suitable for a wide range of applications in various industries.
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Lab products found in correlation
5977a series msd, by Agilent Technologies (3 mentions)
Db 17ht column, by Agilent Technologies (3 mentions)
Agilent 6530 accurate mass q tof, by Agilent Technologies (1 mentions)
Agilent 1290 infinity uplc, by Agilent Technologies (1 mentions)
Masshunter software, by Agilent Technologies (1 mentions)
Masshunter qualitative analysis b 06, by Agilent Technologies (1 mentions)
Masshunter qualitative analysis, by Agilent Technologies (1 mentions)
6 protocols using 7890b series gc
1
GC-MS Analysis of Derivatized Lipids
2
Quantification and Identification of Bisphenol Transformation Products
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Kinetic experiment samples were analysed using a 7890B series GC coupled to an Agilent 7000 series triple quad MS/MS, (Agilent Technologies, USA) 24 (link) . The analysis is described in detail in SI-3.4.1, Chapter 3. Information on quantification, retention times (RT) and MS/MS optimised conditions are summarised in Table SI-3, while matrix-matched validation using isotopically labelled internal standards was performed as described in SI-3.5. Limits of quantification (LOQ) for BPF and BPS were 0.02 or 0.1 mg L -1 , respectively, and the average concentrations of analytes in the blanks were < LOQ.
Non-target screening for the identification of the formed BTPs was conducted using an Agilent 1290 Infinity UPLC coupled to an Agilent 6530 Accurate-Mass QTOF (Agilent Technologies, USA). An Agilent Jet-Stream electrospray source was operated in negative ionisation mode only since no transformation products of bisphenols were identified in our previous studies. The instrument was run in datadependent acquisition mode with a mass range of 50 to 1000 m/z and a scan rate of 2 scans s -1 for MS and 5 scans s -1 for MS/MS (SI-3.4.2, Chapter 3). MassHunter software (Version B.07.00, Agilent Technologies) was used for data analysis.
Non-target screening for the identification of the formed BTPs was conducted using an Agilent 1290 Infinity UPLC coupled to an Agilent 6530 Accurate-Mass QTOF (Agilent Technologies, USA). An Agilent Jet-Stream electrospray source was operated in negative ionisation mode only since no transformation products of bisphenols were identified in our previous studies. The instrument was run in datadependent acquisition mode with a mass range of 50 to 1000 m/z and a scan rate of 2 scans s -1 for MS and 5 scans s -1 for MS/MS (SI-3.4.2, Chapter 3). MassHunter software (Version B.07.00, Agilent Technologies) was used for data analysis.
3
GC-MS Lipid Profiling Protocol
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All lipids were derivatized to trimethylsilyl ethers using 1:1 (vol: vol) Bis(trimethylsilyl)trifluoroacetamide: pyridine and heating at 70 °C for 1 h before analysis on an Agilent 7890B Series GC. Lipids were separated on a 60 m Agilent DB17HT column (60 m x 0.25 mm i.d. x 0.1 μm film thickness) with helium as the carrier gas at constant flow of 1.1 mL/min and programed as follows: 100 °C for 2 min; then 8 °C/min to 250 °C and held for 10 min; then 3 °C/min to 330 oC and held for 17 min. In all, 2 μL of each sample was injected in splitless mode at 250 °C. The GC was coupled to a 5977 A Series MSD with the ion source at 230 °C and operated at 70 eV in EI mode scanning from 50-850 Da in 0.5 s. Lipids were analyzed using Agilent MassHunter Qualitative Analysis (B.06.00) and identified based on retention time and spectra by comparison to previously confirmed laboratory standards, published spectra31 (link),63 (link),64 (link), and spectra deposited in the American Oil Chemists’ Society (AOCS) Lipid Library (http://lipidlibrary.aocs.org/index.cfm ) or the National Institute of Standards and Technology (NIST) databases.
4
Profiling Sterol Methyltransferase Enzymatic Activity
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Lipids were separated with an Agilent 7890B Series GC equipped with two Agilent DB-17HT columns (30 m × 0.25 mm i.d. × 0.15 μm film thickness) in tandem with helium as the carrier gas at a constant flow of 1.1 ml/min and programmed as follows: 100 °C for 2 min, then 12 °C/min to 250 °C and held for 10 min, then 10 °C/min to 330 °C and held for 17.5 min. 2 μL of each sample was injected in splitless mode at 250 °C. The GC was coupled to an Agilent 5977 A Series MSD with the ion source at 230 °C and operated at 70 eV in EI mode scanning from 50 to 850 Da in 0.5 s. Data were analzyed using Agilent MassHunter Qualitative Analysis. Sterols were identified based on retention time and comparison to previously published spectra and laboratory standards. In vitro reactions for each SMT with both sterol substrates were performed at least twice, and the same sterol products were observed by GC-MS each time.
5
Identifying Odor-Active Compounds in Cheddar Cheese
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An Agilent 7890B series GC coupled with a sniffing system (ODP3) was used to locate odor-active components. The isolates obtained by SPME was injected in splitless mode. The temperatures of the olfactory port and transfer line were kept at 230°C and 250°C, respectively. To avoid potential loss of odor-active compounds, gas chromatography-olfactometry (GC-O) analyses were carried out by 4 well-trained panelists on 2 columns with different polarities. Each panelist evaluated the sample once in 2 time segments of 44 min to avoid fatigue. During the GC-O analysis, the panelists recorded the aroma descriptor and intensity value as well as the time when the aroma compound occurred. If 2 or more panelists detected the aroma, an odor-active location was identified. High-purity nitrogen (99.99%) was applied as the carrier gas of GC-O analysis.
Before the experiments, these panelists had been trained by sniffing 60 reference compounds (prepared as 3-to 5-fold odor thresholds in water or odorless sunflower oil; Burdock, 2010; Gemert, 2011) determined by pre-experiments of Cheddar cheeses.
Before the experiments, these panelists had been trained by sniffing 60 reference compounds (prepared as 3-to 5-fold odor thresholds in water or odorless sunflower oil; Burdock, 2010; Gemert, 2011) determined by pre-experiments of Cheddar cheeses.
6
Fatty Acid Composition and Triacylglycerols Analysis
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1-The determination of fatty acid composition (FAC %) and triacyclglycerols (TAGs %)was carried out by gas chromatography type: Gas chromatograph (GC) with FID -Agilent7890B Series GC Custom under the following conditions:
Fatty acid composition by Gas Chromatography determined by capillary GC method, according to the AOCS Official Method Ce 1c-89, Ce 1f-96, Ce 1e-91.
Preparation of fatty acid methyl ester was done for oils prior to GC analysis by converting the triglycerides and fatty acids of oils to their corresponding methyl esters prior to the analysis by gas chromatography. The official AOCS Official Method Ce 2-66. Was followed in preparation.
Analysis of the triacylglycerols composition (TAGs) of fats and oils was determined by capillary GC method as per ISO /TS 17383 method.
Fatty acid composition by Gas Chromatography determined by capillary GC method, according to the AOCS Official Method Ce 1c-89, Ce 1f-96, Ce 1e-91.
Preparation of fatty acid methyl ester was done for oils prior to GC analysis by converting the triglycerides and fatty acids of oils to their corresponding methyl esters prior to the analysis by gas chromatography. The official AOCS Official Method Ce 2-66. Was followed in preparation.
Analysis of the triacylglycerols composition (TAGs) of fats and oils was determined by capillary GC method as per ISO /TS 17383 method.
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