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Hmvec d cells

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HMVEC-D cells are a primary human cell line derived from human dermal microvascular endothelial cells. They are used for in vitro research and experimentation purposes.

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Lab products found in correlation

Egmtm 2 mv microvascular endothelial cell growth medium 2 bulletkit, by Lonza (1 mentions) Ultraglutamine, by Lonza (1 mentions) Singlequots kit, by Lonza (1 mentions) Mycoalert plus mycoplasma detection kit, by Lonza (1 mentions) Il 17a, by R&D Systems (1 mentions) Tnf α, by R&D Systems (1 mentions)

3 protocols using hmvec d cells

1

HMVEC-d Cell Culture Protocol

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HMVEC-d cells (catalog #: CC-2543 Lonza, Basel, Switzerland) were propagated (passages 4–8) and maintained at 37 °C in humidified air with 5% CO2 in EGMTM 2MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM (catalog #: CC-3202, Lonza, Basel, Switzerland) supplemented with growing factors, antibiotics, and 10% fetal bovine serum according to the manufacturer’s specifications.
Cipitelli M.D., Paiva I.A., Badolato-Corrêa J., Marinho C.F., Fiestas Solórzano V.E., da Costa Faria N.R., de Azeredo E.L., de Souza L.J., da Cunha R.V, & de-Oliveira-Pinto L.M. (2022). Subsets of Cytokines and Chemokines from DENV-4-Infected Patients Could Regulate the Endothelial Integrity of Cultured Microvascular Endothelial Cells. Pathogens, 11(5), 509.
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2

Human Colon Cancer and Endothelial Cell Culture

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Human colon adenocarcinoma HCT116 cell lines expressing wt p53 were provided by B. Vogelstein (The Johns Hopkins Kimmel Cancer Center, Baltimore, MD, USA) and were routinely cultured in RPMI 1640 medium with UltraGlutamine from Lonza (VWR, Carnaxide, Portugal) supplemented with 10% fetal bovine serum (FBS) from Gibco (Alfagene, Lisboa, Portugal). Dermal microvascular endothelial HMVEC-D cells (Lonza, VWR) were cultured in endothelial cell growth basal medium supplemented with SingleQuots™ Kit (Lonza, VWR). Both cells were maintained at 37 °C in a humidified atmosphere of 5% CO2. Cells were routinely tested for mycoplasma infection by using the MycoAlert™ PLUS mycoplasma detection kit (Lonza, VWR).
Ramos H., Calheiros J., Almeida J., Barcherini V., Santos S., Carvalho A.T., Santos M.M, & Saraiva L. (2020). SLMP53-1 Inhibits Tumor Cell Growth through Regulation of Glucose Metabolism and Angiogenesis in a P53-Dependent Manner. International Journal of Molecular Sciences, 21(2), 596.
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3

Monocyte-Endothelial Cell Interactions in Psoriasis

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Monocytes were negatively selected from PBMCs using the Pan-Monocyte kit (Miltenyi, Cologne, Germany). HMVEC-D cells (Lonza, Basel, Switzerland) were cultured in complete media (Lonza) at 37°C, 5% CO2. HMVEC-D cells were stimulated for 4 hours in serum free media at 37°C, 5% CO2 with TNF-α (1ng/ml; R&D, Minneapolis, MN) and IL-17A (100ng/ml; R&D) to mimic a psoriatic and cardiovascular cytokine profile, as published in (33 (link)). The cells then rested for 30 minutes before addition of monocytes. HMVEC-D cells were used at P2-P3 and 90–100% confluence in a 6 well dish. 4–5 million monocytes were plated per well and adhered for 1 hour before harvesting the adherent and supernatant fractions and staining for CD14 and CD16 surface markers.
Golden J.B., Groft S.G., Squeri M.V., Debanne S.M., Ward N.L., McCormick T.S, & Cooper K.D. (2015). Chronic psoriatic skin inflammation leads to increased monocyte adhesion and aggregation. Journal of immunology (Baltimore, Md. : 1950), 195(5), 2006-2018.
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