Western blotting was used to measure the related proteins. The total protein of tracheal tissue and HD11 cells was extracted by the whole-cell lysis method. Primary antibodies for TLR4 (bs-20379R, Bioss, Beijing, China), IκBα (10268-1-AP, Proteintech, Wuhan, China), p-IκBα (bs-2513R, Bioss), p65 (bs-0465R, Bioss), p-p65 (bs-0982R, Bioss), IL-1β (A16288, ABclonal, Wuhan, China), TNF-α (bsm-33207M, Bioss), JNK (bs-20760R, Bioss), p-JNK (bs-17591R, Bioss), ERK (bs-2637R, Bioss), p-ERK (bs-1645R, Bioss), and GAPDH (A5028, bimake, Houston, TX) (all at 1:1,000 dilution) protein were incubated for 12 h at 4°C. Secondary anti-rabbit IgG horseradish peroxidases (bs-0061R, Bioss) were incubated for 1.5 h. Enhanced chemiluminescence (ECL ) reagent (Beyotime, China) was used to visualize the bound immune complexes by automatic chemiluminescence image analysis system (Tanon, China) and the density of the protein bands was measured with Image J software (V 1.42, National Institutes of Health; Hu et al., 2021 ).
Bs 2637r
Manufactured by Bioss Antibodies
The Bs-2637R is a laboratory instrument designed for centrifugation. It features a compact and durable design, providing a reliable and consistent performance for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Bs 2513r, by Bioss Antibodies (1 mentions)
Bs 0465r, by Bioss Antibodies (1 mentions)
Bs 0982r, by Bioss Antibodies (1 mentions)
Bs 0061r, by Bioss Antibodies (1 mentions)
A16288, by ABclonal (1 mentions)
Bs 17591r, by Bioss Antibodies (1 mentions)
Bsm 33207m, by Bioss Antibodies (1 mentions)
Ecl reagent, by Beyotime (1 mentions)
Ab12405, by Abcam (1 mentions)
Ab8898, by Cell Signaling Technology (1 mentions)
Hrp conjugated secondary antibody, by Boster Bio (1 mentions)
Bs 5477r, by Bioss Antibodies (1 mentions)
Bs 3016r, by Bioss Antibodies (1 mentions)
H3k9me3, by Abcam (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
2 protocols using bs 2637r
1
Protein Expression Analysis in Tracheal Tissue and Cells
2
Western Blot Analysis of Cellular Proteins
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Total protein was extracted from cells using RIPA buffer (Beyotime), with an equal amount loaded onto 12% SDS‐PAGE gel. The following primary antibodies were used for Western blot analysis: Suv39h1 (48 kD, 1:800; Abcam, ab12405), proliferating cell nuclear antigen (PCNA, 29 kD, 1:300; Bioss, bs‐0754R), complement C3 (180 kD, 1:200; Santa Cruz, sc20137, CA), phosphor‐p38 (Tyr323) (41 kD, 1:300; Bioss, bs‐5477R), phosphor‐ERK1/2 (Thr202 + Tyr204) (41 kD, 1:300; Bioss; bs‐3016R), p38 (41 kD, 1:300; Bioss, bs‐0637R), ERK1/2 (42/44 kD, 1:300; Bioss, bs‐2637R), histone H3K9 trimethylation (H3K9me3, 15.4 kD, 1:800; Abcam, ab8898) and GAPDH (37 kD, 1:1000; Cell Signaling, #5174). Following the incubation with primary antibodies, HRP‐conjugated secondary antibodies (1:1500‐1:2000; Boster) were used. The primary antibody was diluted with Tris‐buffered saline Tween‐20 (TBST) containing 5% skimmed milk powder.
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