Horseradish peroxidase hrp conjugated affinipure goat anti rabbit igg h l

For WB analysis, anti-p30, -p72, anti-pC315R, and anti-pH359L rabbit sera were raised against recombinant ASFV p30, p72 pC315R, and pH359L proteins and deposited at the African Swine Fever Regional Laboratory (Lanzhou), LVRI, of the Chinese Academy of Agricultural Sciences. Anti-CD2v mouse sera were kindly provided by Liguo Zhang from Institute of Biophysics, Chinese Academy of Sciences. Anti-β-actin (13E5) rabbit monoclonal antibody (catalog no. 4970) and anti-GAPDH (14C10) rabbit monoclonal antibody (catalog no. 2118) were purchased from Cell Signaling Technology. Anti-β-tubulin rabbit polyclonal antibody (catalog no. 10094-1-AP) and horseradish peroxidase (HRP)-conjugated AffiniPure goat anti-rabbit IgG(H+L) (catalog no. SA00001-2) were purchased from ProteinTech Group. Fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG secondary antibody (catalog no. F0382) was purchased from Sigma-Aldrich. A Cell Counting Kit-8 (CCK-8; catalog no. K1018-30) was purchased from APExBIO (USA). TRIzol reagent (catalog no. 15596018), DAPI (4′,6′-diamidino-2-phenylindole; catalog no. 62248), octadecyl rhodamine B (R18, catalog no. O246), and radioimmunoprecipitation assay (RIPA) lysis and extraction buffers (catalog no. 89901) were purchased from Thermo Fisher Scientific. Filters (0.22-μm pore size) were purchased from Millipore.

Ubenimex was provided by Shenzhen Main Luck Pharmaceuticals Inc. (Shenzhen, China). 5-fluorouracil was purchased from Xudong Haipu pharmaceutical Co., LTD. (Shanghai, China). Cisplatin and Oxaliplatin were obtained from Qilu Pharmaceutical Co., Ltd. (Jinan, China). Primary antibodies against human Pim-3, BCL-XL, BCL-2, Bad, and phospho-Bad were purchased from Cell Signaling Technology (Beverly, MA). Antibodies against human CD13, β-actin, P-gp, MRP2, MRP3 and ABCG2 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Horseradish Peroxidase (HRP)-conjugated Affini-pure Goat Anti-Rabbit IgG (H+L) was acquired from Proteintech (Chicago, USA).