Plentiekar2g2

Manufactured by Addgene

The PLentiEKAR2G2 is a plasmid vector designed for lentiviral transduction. It contains an EKA-R2G2 gene, which encodes a genetically-encoded calcium indicator protein. The core function of this product is to facilitate the expression of the EKA-R2G2 calcium sensor in target cells through lentiviral delivery.

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Lab products found in correlation

Gw1 phred, by Addgene (1 mentions) Ptriex rhoa flare, by Addgene (1 mentions) Egfp p65, by Addgene (1 mentions) Pmitotimer, by Addgene (1 mentions) Mcherry lifeact 7, by Addgene (1 mentions) Pgp cmv gcamp6f, by Addgene (1 mentions) Prk5 myc rhoa t19n, by Addgene (1 mentions)

2 protocols using plentiekar2g2

Plasmid Constructs for Molecular Imaging

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The plasmids designed in this study are summarized in Supplementary Table 1. Mammalian vectors used in this study used plasmids designed by us earlier as templates^{16 (link)}. Anti-GFP nanobody vhhGFP4, referred in this paper as “anti-GFP iB” or “iB(GFP)” was from pcDNA3_NSlmb-vhhGFP4, a gift from M. Affolter (Addgene plasmid #35579). pQP-2376 was used as an intermediate cloning step, NSImb domain was further removed, to get pQP-2460. Vector containing PiggyBac^{52 (link)} terminal repeats and EGFP-PAC under CMV promoter, as well as vector for hyperactive PiggyBac transposase expression were constructed by VectorBuilder (Cyagen Biosciences). pLentiEKAR2G2 was a gift from O. Pertz (Addgene plasmid #40178). pcDNA3-AktAR2 was a gift from J. Zhang (Addgene plasmid #64932). GFP-ERK2 was a gift from R. Seger (Addgene plasmid #37145). mEGFP-HRAS plasmid was a gift from K. Svoboda (Addgene plasmid #18662). Anti-RAS monobody NS1, referred in this paper as “iB(RAS)” was synthetized by GenScript, using amino acid sequence from 5E95 Protein Data Bank entry for reverse translation^{53 (link)}. Actin chromobody, referred in this paper as “iB(actin)” is commercially available from ChromoTek.

Redchuk T.A., Karasev M.M., Verkhusha P.V., Donnelly S.K., Hülsemann M., Virtanen J., Moore H.M., Vartiainen M.K., Hodgson L, & Verkhusha V.V. (2020). Optogenetic regulation of endogenous proteins. Nature Communications, 11, 605.

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Comprehensive RhoA Signaling Toolkit

Cited 1 time

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Raichu-RhoA (provided by M. Matsuda [27 (link)]), pTriEx-RhoA FLARE.sc Biosensor WT (RRID:Addgene_12150), pTriEx-RhoA FLARE.sc Biosensor Q63L (RRID:Addgene_12151), pTriEx-RhoA FLARE.sc Biosensor T19N (RRID:Addgene_12152), pRK5-myc-RhoA WT (RRID:Addgene_12962), pRK5-myc-RhoA Q63L (RRID:Addgene_12964), pRK5-myc-RhoA T19N (RRID:Addgene_12963), EGFP-p65 (RRID:Addgene_111190), GW1-pHRed (RRID:Addgene_31473), GW1CMV-Perceval (RRID:Addgene_21737), Laconic/pcDNA3.1 (+) (RRID:Addgene_118627), Pyronic /pcDNA3.1 (+) (RRID:Addgene_51308), pcDNA3.1 FLII12Pglu-700uDelta6 (RRID:Addgene_17866), Cyto-ABKAR (RRID:Addgene_61510), pLentiEKAR2G2 (RRID:Addgene_40178), Kras-Src FRET biosensor (RRID:Addgene_78302), pFRET-HSP33 cys (RRID:Addgene_16076), pGP-CMV-GCaMP6F (RRID:Addgene_40755), mCherry-Lifeact-7 (RRID:Addgene_54491), pMitoTimer (RRID:Addgene_52659), pCLBW cox8 EGFP mCherry (RRID:Addgene_78520).

Socodato R., Rodrigues-Santos A., Tedim-Moreira J., Almeida T.O., Canedo T., Portugal C.C, & Relvas J.B. (2023). RhoA balances microglial reactivity and survival during neuroinflammation. Cell Death & Disease, 14(10), 690.

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