Cck 8 labelling reagent

Manufactured by Dojindo Laboratories

Sourced in Japan

The CCK-8 labelling reagent is a colorimetric assay kit used to measure cell viability and proliferation. The reagent contains a water-soluble tetrazolium salt that can be reduced by dehydrogenase enzymes in viable cells, producing a colored formazan dye that can be quantified spectrophotometrically.

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Lab products found in correlation

Cisplatin, by Merck Group (1 mentions) A33978, by Thermo Fisher Scientific (1 mentions)

2 protocols using cck 8 labelling reagent

Quantifying Cisplatin's Cytotoxicity on HCC Cells

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Briefly, HCC cell lines were seeded onto a 96-well plate (3 × 10³ cells/well) 12 h prior to treatment with various concentrations (0–512 μg/ mL) of cisplatin (Sigma-Aldrich, St. Louis, MO, USA)²⁹. The cells were grown in a final 100-mL volume of culture medium per well. CCK-8 labelling reagent (10 μL; Dojindo Molecular Technologies Inc, Kumamoto, Japan) was added to each well and incubated for 1 h. Absorbance of the formazan product was measured at 450 nm. To achieve significance in quantitative analysis, experiments were repeated at least three times.

Ma H., Xie L., Zhang L., Yin X., Jiang H., Xie X., Chen R., Lu H, & Ren Z. (2018). Activated hepatic stellate cells promote epithelial-to-mesenchymal transition in hepatocellular carcinoma through transglutaminase 2-induced pseudohypoxia. Communications Biology, 1, 168.

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Cell Viability Assay of MDA-MB-231 and MCF-7

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A total of 2×10³ MDA-MB-231 or MCF-7 cells in 100 µl high-glucose DMEM culture solution per well were added into a 96-well plate and incubated overnight. Subsequently, it was tested daily for ≤6 days. Prior to testing, 90 µl serum-free medium with 10 µl CCK-8 labelling reagent (Dojindo, Molecular Technologies, Inc.) was added into each well and incubated for 2 h in the dark at 37°C. The viability of the cells was then analysed by measuring their absorbance at 450 nm using an enzyme–labelled meter (Thermo Fisher Scientific, A33978, Inc.).

Peng J., Pei S., Cui Y., Xia Y., Huang Y., Wu X., Zheng M., Weng M., Han X., Fu H., Yang L., Zhou W., Fu Z., Wang S, & Xie H. (2022). Comparative analysis of transient receptor potential channel 5 opposite strand-induced gene expression patterns and protein-protein interactions in triple-negative breast cancer. Oncology Letters, 24(2), 259.

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