The suspensions of cysts were sedimented by centrifugation and fixed in freshly prepared 2.5% glutaraldehyde in 0.2 M cacodylate buffer (pH 7.2). Specimens were then washed 3 × 15 min in the cacodylate buffer, post-fixed in 2% osmium tetroxide in cacodylate buffer for 2 h at room temperature, and finally washed 3 × 15 min in the same buffer. After dehydration in ascending acetone series, specimens were critical point-dried using CO2, coated with gold. The material was examined and images were acquired at laboratory temperature with JSM-7401F field emission scanning microscope (Jeol, Japan) operating at 4 kV80 (link). Raw TIFF images were assembled using Photoshop CS5 (Adobe, USA). Identical adjustments in brightness and contrast were applied to all images in a given experiment.
Jsm 7401f field emission scanning microscope
Manufactured by JEOL
Sourced in Japan
The JSM-7401F is a field emission scanning electron microscope (FE-SEM) manufactured by JEOL. It is designed to provide high-resolution imaging of a wide range of samples. The JSM-7401F utilizes a field emission electron source to generate a focused electron beam, enabling the microscope to achieve high resolution and low-voltage imaging capabilities.
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Lab products found in correlation
2 protocols using jsm 7401f field emission scanning microscope
1
Scanning Electron Microscopy of Biological Cysts
2
Gastric Tissue Histology and SEM Analysis
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After either 14 (Trial 1) or 21 (Trial 2) days of treatment, control and treated animals were euthanised by cervical dislocation and dissected according to protocols described by Melicherová et al. (2014) . For histological sectioning, gastric tissue was fixed in AFA (Alcohol-Formalin-Acetic Acid) solution and processed according to Valigurová et al. 2008 . The blocks were cut using a Zeiss Hyrax M 300 rotary microtome and the 7 µm thick sections were stained with haematoxylin-eosin. Preparations were viewed using an Olympus BX61 microscope.
For scanning electron microscopy, samples of gastric tissue were fixed overnight at 4 °C in freshly prepared 2.5% glutaraldehyde (v/v) in cacodylate buffer (0.1 M; pH 7.4), washed 3 × 15 min in the buffer, postfixed in 2% OsO 4 in cacodylate buffer for two hour at room temperature, and washed again 3 × 15 min in buffer. After dehydration in a graded acetone series, specimens were critical point-dried using CO 2 , coated with gold, and examined using a JEOL JSM-7401F -Field Emission Scanning Microscope. Abbreviations used in Figs. 123456789: LM -light microscopy; SEM -scanning electron microscopy
For scanning electron microscopy, samples of gastric tissue were fixed overnight at 4 °C in freshly prepared 2.5% glutaraldehyde (v/v) in cacodylate buffer (0.1 M; pH 7.4), washed 3 × 15 min in the buffer, postfixed in 2% OsO 4 in cacodylate buffer for two hour at room temperature, and washed again 3 × 15 min in buffer. After dehydration in a graded acetone series, specimens were critical point-dried using CO 2 , coated with gold, and examined using a JEOL JSM-7401F -Field Emission Scanning Microscope. Abbreviations used in Figs. 123456789: LM -light microscopy; SEM -scanning electron microscopy
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