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15n2 uracil

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[15N2]-uracil is a stable isotope-labeled compound that contains two 15N atoms in the uracil structure. It is used as a labeled substrate for various analytical and research applications.

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Lab products found in correlation

Trimethyl 13c3 caffeine, by Cambridge Isotopes (4 mentions) 13c6 d glucose, by Cambridge Isotopes (4 mentions) 15n indole, by Cambridge Isotopes (4 mentions) 2 15n l lysine dihydrochloride, by Cambridge Isotopes (4 mentions) 13c7 benzoic acid, by Cambridge Isotopes (4 mentions) 15n l tyrosine, by Cambridge Isotopes (4 mentions) 3 3 13c2 cystine, by Cambridge Isotopes (4 mentions) 15n choline chloride, by Cambridge Isotopes (4 mentions) 13c5 l glutamic acid, by Cambridge Isotopes (4 mentions) Formic acid, by Merck Group (3 mentions) 3 4 13c2 cholesterol, by Cambridge Isotopes (3 mentions) 1 2 13c2 palmitic acid, by Cambridge Isotopes (3 mentions) Lc ms water, by Merck Group (3 mentions) Hplc grade acetonitrile, by Merck Group (3 mentions) 2 deoxyguanosine 15n2 13c10 5 monophosphate, by Cambridge Isotopes (2 mentions) Methanol, by Merck Group (1 mentions) Acetyl d3 carnitine, by Cambridge Isotopes (1 mentions) L phenylalanine 13c9, by Cambridge Isotopes (1 mentions) Human serum albumin (hsa), by Merck Group (1 mentions) Ammonium formate, by Fujifilm (1 mentions)

6 protocols using 15n2 uracil

1

Isotopic Standards for Mass Spectrometry

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HPLC grade acetonitrile and methanol, LC-MS water and 98% formic acid were obtained from Sigma-Aldrich (St. Louis, MO). A mixture of 14 stable isotopic chemicals were used as internal standards [18 (link)], including [13C6]-D-glucose, [15N]-indole, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [3,4-13C2]-cholesterol, [15N]-L-tyrosine, [trimethyl-13C3]-caffeine, [15N2]-uracil, [3,3-13C2]-cystine, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [15N]-choline chloride and 2’-deoxyguanosine-15N2,13C10-5’-monophosphate from Cambridge Isotope Laboratories, Inc (Andover, PA).
Teeny S., Jarrell Z.R., Krigbaum N.Y., Cirillo P.M., Go Y.M., Cohn B.A, & Jones D.P. (2023). Third Trimester Serum Amino Acid Metabolism is Associated with Maternal Breast Cancer Diagnosed within 15 years of Pregnancy. Research Square.
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2

Comprehensive Metabolite Quantification Protocol

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HPLC grade acetonitrile and methanol, LC-MS water and 98% formic acid were obtained from Sigma-Aldrich (St. Louis, MO). A mixture of 14 stable isotopic chemicals were used as an internal standard (Go et al. 2015 (link)) included [13C6]-D-glucose, [15N]-indole, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [3,4-13C2]-cholesterol, [15N]-L-tyrosine, [trimethyl-13C3]-caffeine, [15N2]-uracil, [3,3-13C2]-cystine, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [15N]-choline chloride and 2′-deoxyguanosine-15N132, C10-5′-monophosphate from Cambridge Isotope Laboratories, Inc (Andover, PA).
Go Y.M., Weinberg J., Teeny S., Cirillo P.M., Krigbaum N.Y., Singer G., Tran V., Cohn B.A, & Jones D.P. (2023). Exposome epidemiology for suspect environmental chemical exposures during pregnancy linked to subsequent breast cancer diagnosis. Environment international, 178, 108112.
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3

Targeted Metabolomics Stable Isotope Profiling

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HPLC grade acetonitrile and methanol, LC-MS water and 98% formic acid were obtained from Sigma-Aldrich (St. Louis, MO). A mixture of 14 stable isotopic chemicals used as an internal standard [57 (link)] included [13C6]-D-glucose, [15N]-indole, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [3,4-13C2]-cholesterol, [15N]-L-tyrosine, [trimethyl-13C3]-caffeine, [15N2]-uracil, [3,3-13C2]-cystine, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [15N]-choline chloride and 2’-deoxyguanosine-15N2,13C10-5’-monophosphate from Cambridge Isotope Laboratories, Inc (Andover, PA).
Jarrell Z.R., Smith M.R., Hu X., Orr M., Liu K.H., Quyyumi A.A., Jones D.P, & Go Y.M. (2020). Plasma acylcarnitine levels increase with healthy aging. Aging (Albany NY), 12(13), 13555-13570.
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4

Stable Isotopic Internal Standards for LC-MS

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Unless otherwise stated, materials were purchased from Sigma‐Aldrich. A mixture of 11 stable isotopic chemicals used as an internal standard for LC–MS analysis included [13C6]‐d‐glucose, [15N]‐indole, [15N,13C5]‐l‐methionine, [2‐15N]‐l‐lysine dihydrochloride, [15N]‐choline chloride, [13C5]‐l‐glutamic acid, [13C7]‐benzoic acid, [15N]‐l‐tyrosine, [15N2]‐uracil, [3,3‐13C2]‐cystine and [trimethyl‐13C3]‐caffeine purchased from Cambridge Isotope Laboratories, Inc. PyC2‐Gly [(γEC)2G] standard was purchased from CPC Scientific. All metal standards were purchased from either RICCA Chemical Company or Inorganic Ventures.
Jarrell Z.R., Liu K.H., Dennis K.K., Hu X., Martin G.S., Jones D.P, & Go Y. (2023). Discovery of phytochelatins in human urine: Evidence for function in selenium disposition and protection against cadmium. FASEB BioAdvances, 5(9), 367-375.
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5

Stable Isotope Labeled Metabolite Quantification

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Internal standards included the following stable-isotope labeled compounds: [24-13C]-cholic acid, l-Arginine-15N4, Uracil-15N2, l-Tyrosine-13C9,15N, l-Phenylalanine-13C9, 15N, and Acetyl-d3-Carnitine purchased from Cambridge Isotope laboratories; linoleic acid-d11 and eicosapentaenoic acid-d5 purchased from Cayman Chemicals; and [trimethyl-13C3]-caffeine purchased from Milli-poreSigma. Acetonitrile and formic acid (Optima, Fisher Scientific) and isopropyl alcohol (Lichrosolv, MillioporeSigma) used for analysis were LC–MS grade. Water was prepared with an Ultrapure MilliQ system (18 MΩ, Millipore, USA). Purified human hemoglobin (MP Biomedicals), Human Serum Albumin (MilliporeSigma), and Sysmex Reagent Sulfolyser (Henry Schein, Melville, NY) were purchased for the Hb assays.
Yu M., Dolios G., Yong-Gonzalez V., Björkqvist O., Colicino E., Halfvarson J, & Petrick L. (2020). Untargeted metabolomics profiling and hemoglobin normalization for archived newborn dried blood spots from a refrigerated biorepository. Journal of pharmaceutical and biomedical analysis, 191, 113574.
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6

Quantification of 5-FU, Uracil, and Tegafur

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The standards of 5-FU and uracil were purchased from Wako Pure Chemical Ind. Ltd. (Osaka, Japan), and the standard of tegafur was purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). The stable isotope-labeled internal standards (IS) for 5-FU and tegafur; 5-FU-13C,15N2 and tegafur-13C,15N2, respectively, were synthesized by Toronto Research Chemicals (Toronto, Canada) and that for uracil; uracil-15N2, was synthesized by Cambridge Isotope Laboratories Inc. (Andover, MA, USA). Ammonium formate used for preparing working solution was purchased from Wako Pure Chemical Ind. Ltd. (Osaka, Japan). All solvents (water, methanol, acetonitrile, ethyl acetate, and isopropyl alcohol) used in chromatography, mass spectrometry, liquid extraction, and sample preparation were of the highest analytical quality (HPLC or LC/MS grade).
Shiraiwa K., Suzuki Y., Uchida H., Iwashita Y., Tanaka R., Iwao M., Tada K., Hirashita T., Masuda T., Endo Y., Inomata M, & Itoh H. (2021). Simultaneous quantification method for 5-FU, uracil, and tegafur using UPLC-MS/MS and clinical application in monitoring UFT/LV combination therapy after hepatectomy. Scientific Reports, 11, 3132.
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