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3 protocols using glycerol

1

Investigating the Anti-Inflammatory Potential of DIDOX

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LPS was obtained from Sigma-Aldrich. 3,4-Dihydroxy-benzohydroxamic acid (DIDOX) was obtained from MedChem Express. 3-(4,5-dimethyl-2-thiazolyl)-2,5-Diphenyl-2-H-tetrazolium bromide (MTT), and glycerol were purchased from Genview. Dexamethasone (DEX) was purchased from Cisen pharmaceutical company. Fetal bovine serum (FBS) was obtained from Gemini Bio-product. Naphthylethylenediamine and sulfanilamide were obtained from Sinopharm Chemicals. L-glutamine was obtained from Solarbio. Dulbecco’s Modified Eagle’s Medium (DMEM) was obtained from Gibco. In addition to the HPLC was used for chromatography-grade solvents (Tianjin Concord Technology Co., Ltd), other solvents were analytical grade (Tianjin Fuyu Fine Chemical Co., Ltd).
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2

Evaluating Cellular Responses to Oxidative Stress

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Sulforaphane (SF), menadione, digitonin, As(III), and flavin adenine dinucleotide were purchased from Sigma-Aldrich (MO, USA). Cycloheximide (CHX), 4′, 6-diamidino-2-phenylindole (DAPI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), bromophenol blue, glycerol, and bovine serum albumin (BSA) were obtained from Genview (TX, USA). Glucose-6-phosphate dehydrogenase and nicotinamide adenine dinucleoide phosphate (NADP) were purchased from Regal (Shanghai, China). β-Mercaptoethanol was obtained from Dingguo (Beijing, China). Eagle's minimal essential medium (MEM), and RPMI1640 were acquired from Gibco (CA, USA). Fetal bovine serum (FBS) was purchased from Gemini Bio-product (CA, USA). Glucose-6-phosphate and L-glutamine were obtained from Solarbio (Beijing, China).
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3

Biochemicals and Reagents for Cell Experiments

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Sulforaphane (SF), menadione, digitonin, As(III), and flavin adenine dinucleotide were purchased from Sigma-Aldrich (MO, USA). Didox was purchased from MedChemExpress (Monmouth Junction, NJ, USA). Cycloheximide (CHX), 4′,6-diamidino-2-phenylindole (DAPI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), bromophenol blue, glycerol, and bovine serum albumin (BSA) were purchased from Genview (TX, USA). β-Mercaptoethanol was obtained from Dingguo (Beijing, China). Eagle's minimal essential medium (MEM) and RPMI 1640 were acquired from Gibco (CA, USA). Fetal bovine serum (FBS) was purchased from Gemini Bio Products (CA, USA). Glucose-6-phosphate and L-glutamine were obtained from Solarbio (Beijing, China). Glucose-6-phosphate dehydrogenase and nicotinamide adenine dinucleotide phosphate (NADP) were obtained from Regal (Shanghai, China). Solvents used for extraction and isolation were of analytical grade and obtained from Tianjin Fuyu Fine Chemical Company (Tianjin, China). Naphthylethylenediamine and sulfanilamide were obtained from Sinopharm Chemical Reagent Co. Ltd. (China).
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