RNA extraction, RNA retrotranscription, real‐time PCR, and western blot analysis were performed as previously described.36 Membranes were incubated overnight with anti‐NR1D1 (Novus, H00009572‐M02) and anti‐Actin (Sigma, A5441, lot: 061M4808). Image acquisition was performed in a chemiluminescent western blot scanner (Li‐Cor) or exposed on Kodak BioMax Light film. The western blot image presented in Figure 4A was quantified using ImageJ Software (NIH), all other western blot quantifications were performed using the Image Studio Software (Li‐Cor).
Anti nr1d1
Manufactured by Novus Biologicals
Anti‐NR1D1 is a primary antibody that recognizes the nuclear receptor subfamily 1, group D, member 1 (NR1D1) protein. NR1D1 is a transcriptional regulator that is involved in the regulation of circadian rhythms. The Anti‐NR1D1 antibody can be used for the detection and analysis of NR1D1 in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence.
Automatically generated - may contain errors
2 protocols using anti nr1d1
1
Protein Expression Analysis Protocol
2
Comprehensive protein analysis protocol
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