The reference products in this study were selected randomly from 154 lots of EU- and US-sourced reference product. The CellTiter-Blue® and CytoTox-Glo® kits were obtained from Promega (Madison, WI, USA). The phycoerythrin (PE) anti-human CD340 (ErbB2/HER2) antibody was obtained from Biolegend (San Diego, CA, USA). The ErbB2/HER2 enzyme-linked immunosorbent assay (ELISA) kit was obtained from R&D Systems (Minneapolis, MN, USA), the Phospho-Akt (Ser473) ELISA kit was obtained from Cell Signaling Technology (Danvers, MA, USA), and the Human VEGF Quantikine® ELISA kit was obtained from R&D Systems. The CellTrace™ carboxyfluorescein succinimidyl ester (CFSE) Cell Proliferation Kit was obtained from Thermo Fisher Scientific (Waltham, MA, USA), the docetaxel (Taxotere®) was obtained from Sanofi-Aventis (Paris, France), and the PathHunters® Flash Detection Kit was obtained from DiscoverX (Fremont, CA, USA). The recombinant human heregulin-β1 was obtained from Peprotech (Seoul, South Korea) and the Caspase-glo 3/7 assay kit was obtained from Promega.
Recombinant human heregulin β1
Manufactured by Thermo Fisher Scientific
Sourced in United States
Recombinant human heregulin-β1 is a protein produced through recombinant DNA technology. It is a member of the epidermal growth factor (EGF) family and plays a role in the regulation of cell growth and differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Forskolin, by Merck Group (2 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (2 mentions)
Pe anti human cd340 erbb2 her2 antibody, by BioLegend (1 mentions)
Docetaxel taxotere, by Sanofi (1 mentions)
Cytotox glo, by Promega (1 mentions)
Celltrace carboxyfluorescein succinimidyl ester cfse cell proliferation kit, by Thermo Fisher Scientific (1 mentions)
Celltiter blue, by Promega (1 mentions)
Human vegf quantikine elisa kit, by R&D Systems (1 mentions)
Caspase glo 3 7 assay kit, by Promega (1 mentions)
B27 supplement, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Welgene (1 mentions)
Dmem f12, by Merck Group (1 mentions)
Platelet derived growth factor aa, by Thermo Fisher Scientific (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
3 protocols using recombinant human heregulin β1
1
Evaluation of ErbB2/HER2 Biosimilar Candidates
2
Differentiation of T-MSCs into Schwann Cells
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To differentiate T-MSCs into SCs, we used the technique of Razavi et al. [41 (link)] in which human T-MSCs were induced to form neurospheres. We harvested human T-MSCs (80%–90% confluence) and then plated them in plastic dishes at (1.5–2) × 105 cells/cm2 in DMEM/F-12 (Welgene Inc., Daegu, Korea) supplemented with 20 ng/mL basic fibroblast growth factor (bFGF, PeproTech, London, UK), 20 ng/mL epidermal growth factor (EGF, PeproTech), and 2% B27 supplement (1:50, Gibco, Life Technologies, Burlington, ON, Canada) at 37 °C under 5% CO2 in humidified air. We replenished the cultures with fresh medium every 3–4 days. After 7 days, neurospheres were triturated using a 25-gauge needle and replated in laminin-coated cell culture plates containing DMEM/F12 supplemented with 10% FBS, 14 µM forskolin (Sigma-Aldrich), 5 ng/mL platelet-derived growth factor-AA (PDGF, PeproTech), 10 ng/mL bFGF (PeproTech) and 200 ng/mL recombinant human heregulin-β1 (PeproTech) for terminal differentiation. The cells were incubated for 9 days under these conditions, and then harvested for further investigations.
3
Transdifferentiation of Rat ADSCs into Schwann-Like Cells
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ADSCs of Sprague-Dawley rats were purchased from Cyagen Biosciences Inc. (RASMD-01001, Santa Clara, CA, USA). The ADSCs were positive for CD29, CD44 and CD90, but negative for CD34 and CD45. The fourth passage culture of ADSCs was used in this study. For transdifferentiation into Schwann-like cells (Kingham et al., 2007), sub-confluent ADSCs at passage 4 were cultured in serum-free ADSCs growth medium that was supplemented with 1 mM β-mercaptoethanol (Sigma-Aldrich) for 24 hours. After washing with phosphate-buffered saline (PBS; 10 mM, pH 7.4), the cells were incubated in ADSC growth medium containing 35 ng/mL all-trans-retinoic acid (Sigma-Aldrich) for 72 hours. The cells were then washed three times in PBS and incubated in ADSC growth medium. This contained 14 μM forskolin (Sigma-Aldrich), 5 ng/mL platelet-derived growth factor-AA (PeproTech, Rocky Hill, NJ, USA), 10 ng/mL basic fibroblast growth factor (PeproTech) and 200 ng/mL recombinant human heregulin-β1 (PeproTech) for 14 days. The medium was replaced every 3 days.
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