Filipin 3 solution

Manufactured by Merck Group

Filipin III solution is a laboratory reagent used for the detection and visualization of cholesterol and cholesterol-like compounds in biological samples. It is a fluorescent stain that binds specifically to cholesterol, allowing for the identification and localization of cholesterol-rich regions within cells and tissues.

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Lab products found in correlation

Optimal cutting temperature compound, by Sakura Finetek (1 mentions) Prolong diamond antifade mounting medium, by Thermo Fisher Scientific (1 mentions) Filipin 3, by Merck Group (1 mentions) Filipin 3, by BD (1 mentions)

Spelling variants of this product

Filipin (222 citations) Filipin III (104 citations)

2 protocols using filipin 3 solution

Cholesterol Deposition Imaging in Mice Corneas

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Filipin staining was performed to detect any cholesterol deposition in the cornea. Mice from three genotypes were euthanized at the age of four months. Corneas were immediately dissected on ice and embedded in Optimal cutting temperature compound (Sakura Finetek, Torrance, CA) without fixation. Cryosections (10 µm thick) were prepared. Sections were stained at room temperature for 75 minutes with freshly made 0.05 mg/mL filipin III solution (Cat F4767, Sigma, St. Louis, MO). Following staining, sections were mounted in ProLong™ Diamond Antifade mounting medium (Thermo Fisher Scientific, Waltham, MA). Confocal microscopic images of filipin fluorescence were obtained with a Zeiss 780 microscope and Plan-Apochromat 63 ×/1.40 oil objective using 355 nm wavelength for excitation and 371 to 501 nm wavelengths for fluorescence emission. The z-stack obtained contained 3 image slices with a total depth of 1.044 µm.

Dong F., Jin X., Boettler M.A., Sciulli H., Abu-Asab M., Del Greco C., Wang S., Hu Y.C., Campos M.M., Jackson S.N., Muller L., Woods A.S., Combs C.A., Zhang J., Nickerson M.L., Kruth H.S., Weiss J.S, & Kao W.W. (2018). A Mouse Model of Schnyder Corneal Dystrophy with the N100S Point Mutation. Scientific Reports, 8, 10219.

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Quantifying Cholesterol Accumulation in ARID1A-Deficient Cells

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Cellular endogenous cholesterol was assayed by both immunofluorescence staining and flow cytometry. To examine the accumulation of cholesterol inside OVCA429 cells in response to ARID1A knockout, cells were seeded into 24-well plate at a density of 5 x 10⁴ cells/well and cultured overnight. Cells were fixed with 4% paraformaldehyde and washed with cholesterol detection wash buffer (Abcam, Cat#: ab133116). Filipin III solution (Sigma-Aldrich, Cat#: 500 μg/mL) was added into each well for 40 min in the dark. After mounting, cholesterol staining was examined using Leica confocal microscope under excitation of 340–380 nm and emission of 385–470 nm. Red-Dot staining (Biotium, Cat#: 40061) was used to visualize the nucleus. To measure the cholesterol level by flow cytometry, cells were stained with 100 μg/mL Filipin III (Sigma-Aldrich, Cat#: F4767) for 40 min at room temperature. Cells were then washed twice and analyzed by flow cytometry (BD Biosciences, LSRII) for Filipin III at 355 nm laser.

Burton J.L, & Solomon M.J. (2000). Hsl1p, a Swe1p Inhibitor, Is Degraded via the Anaphase-Promoting Complex. Molecular and Cellular Biology, 20(13), 4614-4625.

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