Antibodies used in this study were: anti-MERVL-gag (A-2801, Epigentek), anti-Pou5f1 (sc-5279; Santa Cruz Biotechnology), anti-Nanog (A300-397A, Bethyl), anti-Tubulin (12G10, DSHB), anti-Ezh2 (5246, Cell Signaling), anti-H3K4me3 (ab8580, Abcam), anti-H3K27me3 (9733, Cell Signaling), anti-Flag (F1804, Sigma-Aldrich), anti-Mcm2 (ab4461, Abcam), and anti-Flag (F1804, Sigma).
A 2801
Manufactured by Epigentek
Sourced in United States
The A-2801 is a high-quality laboratory equipment designed for general use in scientific research and analysis. It provides a reliable and consistent means of performing various laboratory tasks. The core function of the A-2801 is to facilitate the efficient and precise execution of experimental procedures in a controlled environment.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using a 2801
1
Antibody Validation for Stem Cell Research
2
Immunofluorescence Staining of Embryonic Stem Cells
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ESCs were fixed in 4% of formaldehyde for 10 min at room temperature (RT), then washed three times with PBS and blocked for 1 h with 5% normal goat serum (NGS) in PBS containing 0.1% Triton X-100 (PBST) at RT. Primary antibody was diluted in PBST containing 1% NGS and incubated with cells overnight at 4°C. After incubation, cells were washed once with PBST and incubated with fluorophore-labeled secondary antibodies for 1 h at RT. DNA/nuclei were counterstained with DAPI. Immunofluorescence images were recorded by Olympus FV1000 confocal system. The primary antibodies used for immunofluorescence were anti-Oct4 (1:100, sc-5279; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-MERVL-Gag (1:100, A-2801, Epigentek, Farmingdale, NY, USA).
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