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Te buffer agar gels

Manufactured by Thermo Fisher Scientific
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TE buffer AGAR gels are a type of laboratory equipment used for electrophoresis. They are prepared by dissolving agarose in a Tris-EDTA (TE) buffer solution. The resulting gel matrix is used to separate and analyze DNA, RNA, or protein samples based on their size and charge.

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Lab products found in correlation

Dl600, by Keygen Biotech (2 mentions)

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TE buffer (73 citations) Agar Gel (1 citations)

2 protocols using te buffer agar gels

1

Characterizing circZNF609 cDNA and gDNA

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The complementary DNA (cDNA) and genomic DNA (gDNA) PCR products of circZNF609 were determined using TE buffer AGAR gels (Thermo Fisher Scientific). DL600 (KeyGen, Nanjing, China) was set as DNA marker. The electrophoretic voltage (110 V) was adopted to separate the DNA half an hour, followed by UV irradiation.
He Y., Huang H., Jin L., Zhang F., Zeng M., Wei L., Tang S., Chen D, & Wang W. (2020). CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions. Cell Death & Disease, 11(5), 358.
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2

Electrophoretic Separation of circ-GLI1

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Through TE buffer AGAR gels from Thermo Fisher Scientific (Waltham, MA, USA), cDNA and gDNA PCR products of circ-GLI1 were tested. As the DNA markers, DL600 (KeyGen, Nanjing) was adopted. Electrophoretic voltage (110V) was performed to isolate DNA. Ultraviolet radiation was applied to determine results. All samples were assayed in triplicate.
Chen J., Zhou X., Yang J., Sun Q., Liu Y., Li N., Zhang Z, & Xu H. (2020). Circ-GLI1 promotes metastasis in melanoma through interacting with p70S6K2 to activate Hedgehog/GLI1 and Wnt/β-catenin pathways and upregulate Cyr61. Cell Death & Disease, 11(7), 596.
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