Aav8 tbg cre

Manufactured by Addgene

AAV8-TBG-Cre is an adeno-associated virus (AAV) vector. The vector contains the Cre recombinase gene under the control of the TBG (thyroxine-binding globulin) promoter.

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Lab products found in correlation

Protease inhibitor cocktail, by G Biosciences (1 mentions) L lysine, by Cambridge Isotopes (1 mentions) Protein assay dye regent, by Bio-Rad (1 mentions) L glutamine, by Cambridge Isotopes (1 mentions) L methionine, by Cambridge Isotopes (1 mentions) L glutamic acid, by Cambridge Isotopes (1 mentions) 0.45 μm nitrocellulose membrane, by Bio-Rad (1 mentions) L tryptophan, by Cambridge Isotopes (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) N methyl n trimethylsilyl trifluoroacetamide, by Merck Group (1 mentions) L valine, by Cambridge Isotopes (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions)

3 protocols using aav8 tbg cre

Metabolomic Analysis of Transgenic Mice

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C57bl6/j mice were obtained from Jackson Laboratories (Stock #000664). Labeled internal standards citric acid (2,2,4,4-D4, 98%; #DLM-3487), succinic acid (2,2,3,3-D4, 98%; #DLM-584), l-valine (2,3,4,4,4,5,5,5-D8; 98%; #DLM-311), l-glutamic acid (¹³C5, 99%; #CLM-1800), l-glutamine (¹³C5, 99%, #CLM-1822), l-lysine (¹³C6, 99%; #CLM-2247), l-methionine (¹³C5, 99%; #CLM-893), and l-tryptophan (¹³C11, 99%; #CLM-4290) and tracer glucose (¹³C6, 99%, #CLM-1396) were obtained from Cambridge Isotope Laboratories, Inc. AAV8-TBG-NULL (#105536-AAV8) and AAV8-TBG-Cre (#107787-AAV8) were purchased from Addgene. Pre-filled bead mill tubes containing 1.4 mm ceramic beads (#15-340-153) were purchased from FisherScientific. Methoxaymine hydrochloride (#226904, MOX) and N-methyl-N-(trimethylsilyl) trifluoroacetamide (#694709, MSTFA) were purchased from Sigma Aldrich. Protease inhibitor cocktail (#786-437) was purchased from G Biosciences. Bio-Rad protein assay dye regent (#5000006) and 0.45 μm nitrocellulose membrane (#1620115) were purchased from Bio-Rad.

Rauckhorst A.J., Borcherding N., Pape D.J., Kraus A.S., Scerbo D.A, & Taylor E.B. (2022). Mouse tissue harvest-induced hypoxia rapidly alters the in vivo metabolome, between-genotype metabolite level differences, and 13C-tracing enrichments. Molecular Metabolism, 66, 101596.

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Hepatocyte-specific Pdia1 deletion in mice

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C57BL/6 mice with Pdia1 floxed alleles were obtained from Dr. J Cho (Univ. of Illinois-Chicago) [60 (link)]. To induce hepatocyte-specific Pdia1 deletion, mice were transduced with Ad-Cre [63 ] (0.9x 10¹⁰ pfu/mouse) or AAV8-TBG-Cre (Addgene, Watertown, MA) (1x 10¹¹ VG/mouse) at the age of 6–10 wks. Littermates transduced with Ad-βGal [63 ] (0.9x10¹⁰ pfu/mouse) or AAV8-TBG-βGal (Addgene) (1x 10¹¹ VG/mouse) were used as controls. Experiments were performed 6–11 wks post-transduction. All mice were fed with regular mouse chow-diet. Both male and female mice were used in the study. All procedures were performed by protocols and guidelines reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) at the SBP Medical Discovery Institute (AUF # 20–056).

Chen Z., Wang S., Pottekat A., Duffey A., Jang I., Chang B.H., Cho J., Finck B.N., Davidson N.O, & Kaufman R.J. (2024). Conditional hepatocyte ablation of PDIA1 uncovers indispensable roles in both APOB and MTTP folding to support VLDL secretion. Molecular Metabolism, 80, 101874.

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Investigating Cre-Mediated Recombination in Mice

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Rosa^YFP/YFP mice (The Jackson Laboratory, Bar Harbor, ME) were crossed with C57BL/6J (The Jackson Laboratory, Bar Harbor, ME) mice to generate Rosa^YFP/+ mice [22 (link)]. AAV8-CMV-red fluorescent protein (RFP), AAV8-CMV-Cre, AAV8-TBG-LacZ (encodes β-galactosidase), and AAV8-TBG-Cre viral preps were produced by Addgene (Watertown, MA; Addgene viral prep numbers: 105548-AAV8, 105537-AAV8, 105534-AAV8, and 107787-AAV8, respectively) using plasmids gifted by Dr. James M. Wilson to Addgene. AAV8 vectors were diluted in saline to a total volume of 50uL. Rosa^YFP/+ mice were given intraperitoneal injections of 2.0 × 10¹¹ genome copies at P2 with the day of birth defined as P0 [23 (link),24 (link)]. Tissues were harvested 8 days and 54 days after injection, at P10 and P56, respectively. Both male and female neonates were included in the experiment: (a) AAV8-CMV-RFP: 6 males and 4 females were analyzed at P10; (b) AAV8-CMV-Cre: 3 males and 8 females were analyzed at P10, and 2 males and 3 females were analyzed at P56; (c) AAV8-TBG-LacZ: 3 males and 4 females were analyzed at P10; (d) AAV8-TBG-Cre: 2 males and 6 females were analyzed at P10, and 6 males and 1 female were analyzed at P56. The protocol was approved by the Institutional Animal Care and Use Committee of the Cincinnati Children’s Hospital Medical Center (IACUC2018-0074, approved 9 November 2018).

Lee S., Zhou P., Whyte S, & Shin S. (2020). Adeno-Associated Virus Serotype 8-Mediated Genetic Labeling of Cholangiocytes in the Neonatal Murine Liver. Pharmaceutics, 12(4), 351.

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