Cells were collected and resuspended in RIPA buffer (Thermo Fisher Scientific) with added protease inhibitors (Roche). Protein concentration was quantified using the Bio-Rad DC protein assay (15 µg loaded per well). Protein samples were resuspended in Laemmli buffer and separated on SDS-PAGE and transferred onto PVDF membranes. Antibodies used included anti–β-actin 13E5 (1:5,000; Cell Signaling Technology), anti–E-cadherin HECD-1 (1:200; Abcam), anti–DDR1 C-20 (1:200; Santa Cruz Biotechnology), anti-KIFC1 (HSET; 1:500; Bethyl Laboratories), anti-Mad2 (1:500; Bethyl Laboratories), anti-RhoE (1:100; Sigma-Aldrich), anti-p190 (1:250; BD Biosciences), anti–STARD8 E-2 (DLC3; 1:100; Santa Cruz Biotechnology), anti–N-cadherin (1:500; BD Biosciences), anti–Vimentin RV202 (1:500; BD Biosciences), anti-ERM (1:500; Cell Signaling Technology), anti–pMLC T18/S19 (1:500; Cell Signaling Technology), and anti–pDDR1 Tyr513 (1:100; Origene). Western blots were developed using a SRX-101A Konica Minolta and scanned.
Anti p190
Manufactured by BD
Anti-p190 is a laboratory equipment product. It is used for the detection and analysis of the p190 protein. The core function of Anti-p190 is to facilitate the identification and quantification of the p190 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Anti e cadherin hecd 1, by Abcam (1 mentions)
Anti β actin 13e5, by Cell Signaling Technology (1 mentions)
Anti mad2, by Fortis Life Sciences (1 mentions)
Srx 101a, by Konica Minolta (1 mentions)
Anti p ddr1 tyr513, by OriGene (1 mentions)
Anti erm, by Cell Signaling Technology (1 mentions)
Anti vimentin rv202, by BD (1 mentions)
Dc protein assay, by Bio-Rad (1 mentions)
Anti n cadherin, by BD (1 mentions)
Anti actin c 2, by Santa Cruz Biotechnology (1 mentions)
Anti n wasp, by Cell Signaling Technology (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Odyssey fc imaging system, by LI COR (1 mentions)
2 protocols using anti p190
1
Western Blotting Protocol for Protein Analysis
2
Quantitative Analysis of miR-142-3p Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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iHMVEC cells (7 × 105 cells/10 cm2 dish) were transfected with 100 pmol of control 1, miR-142-3p and miR-142-3p−1 mirVana miRNA mimics using Lipofectamine RNAiMAX (Life Technologies). Two days after transfection, cells were harvested in 20 mM Tris pH 7.5, 100 mM NaCl, 1% Triton X-100, 10% glycerol, 1 mM EDTA, and Complete EDTA-free protease inhibitors (Roche). Quantitative Western blotting using the Odyssey Fc Imaging System (LI-COR Biosciences) was performed as described (Manzano et al. 2013 (link)) using anti-p190 (BD Biosciences, Cat. 610149), anti-cofilin 2 (EMD Millipore, Cat. 07-300), anti-N-WASP (30D10, Cell Signaling Technology), and anti-actin (C-2, Santa Cruz Biotechnology) antibodies.
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