For assessing adsorption efficiency of both NP formulations, UV-visible spectrophotometry was done using a Multiskan SkyHigh UV/VIS Spectrophotometer (Thermo Fisher Scientific). Solutions of both NP formulations and Au-complex of known concentrations were prepared, spectra were recorded and calibration curves were obtained. Through the formula for calculating the concentration of unknown samples, the authors obtained the percentage of Au-complex in formulations A and B.
Multiskan skyhigh uv vis spectrophotometer
Manufactured by Thermo Fisher Scientific
The Multiskan SkyHigh UV/VIS Spectrophotometer is a laboratory instrument designed to measure the absorbance or transmittance of light in the ultraviolet and visible wavelength ranges. It is capable of performing spectrophotometric analysis on a variety of samples, such as proteins, nucleic acids, and other biological and chemical compounds.
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2 protocols using multiskan skyhigh uv vis spectrophotometer
1
Quantifying Gold Nanoparticle Adsorption
2
Stability of Dendrimer Nanoparticles with Au-complex
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The stability of CMCht/PAMAM dendrimer NPs + Au-complex formulations was analyzed using a UV-visible spectrophotometer. The stability was evaluated in terms of ionic strength and pH. The effect of ionic strength was evaluated 24 h after the addition of a solution of NaCl with final concentrations of 0, 0.001, 0.01, 0.1 and 0.5 M.
The effect of pH was evaluated 24 h after adjusting the pH to 2, 4, 6, 8 and 10. The absorption spectra of freshly prepared, concentrated solutions of each type of NP were recorded using a Multiskan SkyHigh UV/VIS Spectrophotometer (Thermo Fisher Scientific).
The effect of pH was evaluated 24 h after adjusting the pH to 2, 4, 6, 8 and 10. The absorption spectra of freshly prepared, concentrated solutions of each type of NP were recorded using a Multiskan SkyHigh UV/VIS Spectrophotometer (Thermo Fisher Scientific).
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