The genomic DNA of Enterococcus faecalis strain ATCC19433 was extracted utilizing a Tiangen Bacterial Genomic DNA Extraction Kit (Tiangen Biotech) following the manufacturer's instructions. The puri ed genomic DNA was quanti ed with a TBS-380 uorometer (Turner BioSystems Inc., Sunnyvale, CA). Only DNA samples meeting high-quality criteria, including no degradation, OD260/280 = 1.8 ~ 2.0, and a quantity of > 1 µg were utilized for subsequent analyses.
Tbs 380 uorometer
Manufactured by Promega
The TBS-380 Fluorometer is a versatile laboratory instrument used to measure the fluorescence intensity of samples. It is designed to provide accurate and reliable fluorescence measurements across a wide range of applications. The core function of the TBS-380 Fluorometer is to detect and quantify the fluorescent properties of various substances, enabling researchers and scientists to analyze and characterize their samples.
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2 protocols using tbs 380 uorometer
1
Enterococcus faecalis DNA Extraction
2
Genomic DNA Isolation from Bacteria
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A total of 5 ml collected broth from YEME liquid medium was centrifuged at 4000 g for 10 min. Genomic DNA was isolated from the cell pellets using the ChargeSwitch® gDNA Mini Bacteria Kit (Life Technologies) according to the manufacturer's instructions. Puri ed genomic DNA was quanti ed using a TBS-380 uorometer (Turner BioSystems Inc., Sunnyvale, CA). High quality DNA (OD260/280 = 1.8 ~ 2.0, > 10 µg) was used for further research.
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