Sep pak accell plus qma carbonate

All reagents and solvents, unless specified otherwise, were purchased from Sigma Aldrich (Zwijndrecht, Netherlands) and VWR International (Amsterdam, Netherlands), and used without further purification. All materials were purchased from VWR International (Amsterdam, Netherlands), unless specified otherwise. Saline solution for injection (0.9% NaCl w/v%) and water for injection were acquired from B. Braun (Melsunger, Germany). The cassette was prepared in-house, starting from a commercially available [¹⁸F]FDOPA cassette from Trasis (Ans, Belgium). The GMP-grade precursor to [¹⁸F]DPA-714, DPA-714 tosylate, was purchased from Pharmasynth (Tallinn, Estonia). Sep-Pak Accell Plus QMA carbonate and Sep-Pak C18 Plus cartridges were purchased from Waters Corporation (Etten-Leur, Netherlands). Fluorine-18 for labelling was obtained on-site using a Siemens Eclipse HP cyclotron, in the form of [¹⁸O]H₂O-bound [¹⁸F]fluoride via a ¹⁸O(p,n)¹⁸F nuclear reaction in a tantalum target. ¹⁸O-enriched water (≥97%) was purchased from Rotem Industries (Dimona, Israel). Cyclotron-specific parts are described in “Cyclotron” section.

Tracepur H₂O and Kryptofix222 (K₂₂₂) were purchased from Merck. Anhydrous acetonitrile (MeCN), dimethyl sulfoxide (DMSO), oxalic acid and n-octanol were obtained from Sigma-Aldrich; ethanol (EtOH), sodium dihydrogen phosphate (NaH₂PO₄) and disodium hydrogen phosphate (Na₂HPO₄) were obtained from Carl Roth; 0.9% sodium chloride (NaCl)-solution was obtained from VWR (Bruchsal, Germany). Aqueous [¹⁸F]fluoride solution was purchased from EuroPET in Freiburg or the University Hospital Tübingen. The Sep-Pak Accell Plus QMA Carbonate (46 mg) and Sep-Pak C18 Plus SPE Light cartridges (130 mg) were obtained from Waters (Eschborn, Germany). For radioanalytical use, a Dionex UltiMate 3000 system equipped with a Raytest Gabi Star radioactivity detector was used together with a Chromolith Performance (RP-18e, 100–4.6 mm, Merck, Germany) column. All operations were performed with a flow rate of 4 mL/min using H₂O + 0.1% TFA and MeCN + 0.1% TFA as solvents. Radioactivity was measured by an ISOMED 2010 activimeter.