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Celltiter 96 well assay reagent

Manufactured by Promega
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Sourced in United States

The CellTiter 96-well assay reagent is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. It measures the conversion of a tetrazolium salt into a colored formazan product by metabolically active cells.

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Lab products found in correlation

Gemcitabine hydrochloride, by Fujifilm (1 mentions)

Close spelling variants of this product

CellTiter 96 (150 citations) CellTiter 96 reagent (13 citations)

2 protocols using celltiter 96 well assay reagent

1

Cell Proliferation Assay Protocol

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A total of 5.0 × 10 3 cells were passaged into 96-well plates and cultured in 100 μL of medium supplemented with 10% FBS and 1% penicillin. MTS assays were conducted using the CellTiter 96-well assay reagent (cat: G358B; Promega, Madison, WI, USA) according to the manufacturer's protocol.
Miyazaki K., Ariake K., Sato S., Miura T., Xun J., Douchi D., Ishida M., Ohtsuka H., Mizuma M., Nakagawa K., Kamei T, & Unno M. (2024). GFPT2 expression is induced by gemcitabine administration and enhances invasion by activating the hexosamine biosynthetic pathway in pancreatic cancer. Clinical & experimental metastasis.
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2

Gemcitabine Cytotoxicity Assay

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Cells were plated at 5×103 cells/well in 96-well plates. After 24 h, the medium was replaced by another containing from 1×10−4 to 1×103 µM gemcitabine hydrochloride (FUJIFILM WAKO Pure Chemical Corporation). Cells were cultured for 120 h and then cell viability was measured by MTS assays using the CellTiter 96-well assay reagent (Promega Corporation), as recommended by the manufacturer. The absorbance was measured at a wavelength of 490 nM. Each test was carried out in triplicate.
Chao D., Ariake K., Sato S., Ohtsuka H., Takadate T., Ishida M., Masuda K., Maeda S., Miura T., Mitachi K., Yu X.J., Fujishima F., Mizuma M., Nakagawa K., Morikawa T., Kamei T, & Unno M. (2021). Stomatin-like protein 2 induces metastasis by regulating the expression of a rate-limiting enzyme of the hexosamine biosynthetic pathway in pancreatic cancer. Oncology Reports, 45(6), 90.
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