Primary antibodies used in this study: Rabbit polyclonal anti‐human FBN1 (HPA021057, used in immunofluorescence staining of MG63 cells; Sigma‐Aldrich), mouse monoclonal anti‐FN (610078; BD), mouse monoclonal anti‐RECK (5B; Takahashi et al., 1998 ), mouse monoclonal anti‐integrin β1 (CD29; 610467; BD), mouse monoclonal anti‐integrin α2 (611016; BD), mouse monoclonal anti‐integrin α5 (CD49e: 610633; BD), mouse monoclonal anti‐FBN (pan; MAB2641: clone689, used in immunoblot assay, recognizes both FBN1 and FBN2; Merck Millipore), rabbit polyclonal anti‐MMP14/MT1‐MMP (EP1264Y: ab51074; Abcam), rabbit polyclonal anti‐ADAMTS10 (Matsuzaki et al., 2018 ), mouse monoclonal anti‐α‐tubulin (DM1A; Merck), and polyclonal horseradish peroxidase (HRP)‐conjugated goat anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH; [V‐18] HRP; sc‐20357 HRP; Santa Cruz Biotechnology). Secondary antibodies: Anti‐rabbit immunoglobulin G (IgG)‐HRP (ab6721; Abcam), anti‐mouse IgG‐HRP (A4416; Sigma‐Aldrich), anti‐mouse IgG‐CF488 (20018; Biotium), and anti‐rabbit IgG‐CF647 (20282; Biotium).
Anti rabbit igg cf647
Manufactured by Biotium
Sourced in United States
Anti-rabbit IgG-CF647 is a secondary antibody conjugated with a fluorescent dye, CF647. It is designed to detect and visualize rabbit primary antibodies in various immunoassays and imaging applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse igg cf488, by Biotium (2 mentions)
Anti mouse igg hrp, by Merck Group (1 mentions)
Ab6721, by Abcam (1 mentions)
Cd49e, by BD (1 mentions)
Ep1264y, by Abcam (1 mentions)
Hpa021057, by Merck Group (1 mentions)
Ab51074, by Abcam (1 mentions)
Anti rabbit igg hrp, by Abcam (1 mentions)
Anti mouse igg hrp a4416, by Merck Group (1 mentions)
2 protocols using anti rabbit igg cf647
1
Antibodies and Immunostaining Protocol
2
Antibody Characterization for ADAMTS10 and RECK
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Rabbit polyclonal anti-ADAMTS10 antibodies (pAb-867) were raised against a synthetic oligopeptide (SGHSKLPKRQRAC; in the second TSR domain). Other primary antibodies: mouse monoclonal antibodies against RECK (5B11D12) (Takahashi et al., 1998 (link)), FN (610078, BD), MT1-MMP (ab51074; Abcam) and α-tubulin (DM1A; Calbiochem/Merck Millipore, Burlington, USA). Secondary antibodies: anti-rabbit IgG-HRP (ab6721; Abcam), anti-mouse IgG-HRP (A4416; Sigma-Aldrich), anti-mouse IgG-CF488 (20018; Biotium, Fremont, USA), anti-mouse IgG-CF405M (20182, Biotium) and anti-rabbit IgG-CF647 (20282: Biotium). Antibody dilution: primary 1:1000 and secondary 1:20000 for immunoblot assays, primary 1:300 and secondary 1:1000 for immunofluorescence staining. Specificity of pAb-867 and 5B11D12 in immunofluorescence staining was assessed in MG-63 cells genetically manipulated to suppress (ADAMTS10) or inactivate (RECK) the expression of endogenous target genes (see Fig. S2 ).
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