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2 protocols using e coli cmcc b 44103

1

Antibacterial Efficacy Evaluation Protocol

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Agar, gentamicin sulfate (AR), ethylene glycol (AR), sodium cyanoborohydride and sodium periodate (AR) were all purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). E. coli (CMCC(B) 44103) and S. aureus (ATCC 25923) were all obtained from China Center of Industrial Culture Collection, Beijing, China. Tryptic soy Agar (TSA) and Tryptone soya broth (TSB) were obtained from Beijing Land Bridge Technology Co., Ltd. (Beijing, China).
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2

Chitosan-Based Antimicrobial Biomaterial

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Chitosan (CS, viscosity < 200 mPa.s, 97.8% deacetylated), N-acetyl-L-cysteine (NAC), 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC), deoxycholic acid (DA), and succinic anhydride (SA) were purchased from Macklin (Shanghai, China). Both E. coli (CMCC(B) 44103) and S. aureus (ATCC 25923) were from the China Center of Industrial Culture Collection, Beijing, China. In addition, methanol, ammonia, glacial acetic acid, ethanol, acetone, and DMSO were from Guanghua Technology Co., Ltd. (Shantou, China). The reagents and chemicals were all of analytical grade and were not further purified. All experiments used ultrapure water.
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