Frozen ear tissue was embedded in O.C.T compound (Fischer HealthcareTM Tissue PlusTM) cryosectioning. The tissues were sectioned into 5 µm thick sections using Thermo Scientific Cryotome FE. Geimsa stain (Sigma Aldrich, Catalog No: GS) was used to analyze mast cells. The Geimsa stain nuclei in varying shades of purple and the cytoplasm is stained blue to light pink. Eosinophils and red blood cells are stained shades of pink and bright orange, whereas mast cell granules are stained purple. The sections were imaged at 40X magnification on a Nikon Eclipse E800 microscope and RT3 camera/spot advanced software (version 4.6). With the help of a dermatopathologist (Dr. Glynis Scott, URMC-Department of Dermatology), the number of mast cells (intact vs. de-granulated) were counted in each section. The sections were also stained with hematoxylin and eosin stain (H&E) using standard procedures and the sections were imaged at 40X magnification (Nikon Eclipse E800).
Geimsa stain
Manufactured by Merck Group
Sourced in Germany
Geimsa stain is a laboratory stain used to differentiate and identify various cellular components, such as nuclei, cytoplasm, and cell membranes, in microscopic preparations. It is a common staining technique employed in hematology, parasitology, and other areas of biological research and diagnosis.
Automatically generated - may contain errors
Lab products found in correlation
Cryotome fe, by Thermo Fisher Scientific (1 mentions)
Eclipse e800, by Nikon (1 mentions)
Gentamicin sulfate, by Merck Group (1 mentions)
Sorbitol, by Merck Group (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Alanine aminotransferase, by Randox (1 mentions)
Aspartate aminotransferase, by Randox (1 mentions)
2 protocols using geimsa stain
1
Quantifying Mast Cell Populations in Frozen Tissue
2
Cell Culture and Biochemical Assays
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RPMI 1640 was purchased from GIBCO, gentamicin sulfate, sorbitol and geimsa stain was purchased from Sigma Aldrich, Germany, while aspartate aminotransferase, albumin, alanineaminotransferase, alkaline phosphate, protein and albumin kit were purchased from Randox, London.
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