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Af488 labeled secondary goat anti rabbit antibody

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The AF488-labeled secondary goat anti-rabbit antibody is a fluorescently-labeled antibody used to detect the presence of rabbit primary antibodies in various experimental techniques, such as immunohistochemistry, flow cytometry, and Western blotting. The antibody is conjugated with the Alexa Fluor 488 dye, which emits green fluorescence when excited with a suitable light source, allowing for the visualization and localization of the target proteins.

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Lab products found in correlation

Sp5 inverted 2 photon flim confocal, by Leica (2 mentions) Mouse anti human gbp1 5 antibody, by Santa Cruz Biotechnology (2 mentions) Qdot 605 labeled secondary goat anti mouse antibody, by Thermo Fisher Scientific (2 mentions)

2 protocols using af488 labeled secondary goat anti rabbit antibody

1

Immunofluorescence Imaging of GBP1-5 and HSP90

Check if the same lab product or an alternative is used in the 5 most similar protocols
A375 cells mounted on cover slips were treated with IFNγ for 24 hours. After washing 2 times with PBS, cells were fixed with 4% PFA for 15 minutes and washed 2 times with PBS for 5 minutes each. Then, the cells were permeabilized with 0.5% triton x-100 in PBS for 10 minutes and rinsed 2 times with PBS for 5 minutes each. Antigens were blocked with 10% normal goal serum in PBS for 30 minutes. Then, primary antibodies were added at 1:50 dilutions of mouse anti-human GBP1-5 antibody (Santa Cruz, 166960) or rabbit anti-human HSP90 antibody (CST, 4877), and incubated at 4°C overnight. Afterwards, the cells were washed and incubated with 1:500 dilutions of Qdot 605 labeled secondary goat anti-mouse antibody (Thermo Fisher, Q11002) or AF488-labeled secondary goat anti-rabbit antibody (Thermo Fisher, A11034), and then mounted on glass slides with ProLong Gold reagent containing DAPI. Confocal fluorescence images were collected using a 63X oil-immersion objective (Leica SP5 Inverted 2-Photon FLIM confocal).
Li G., Kryczek I., Nam J., Li X., Li S., Li J., Wei S., Grove S., Vatan L., Zhou J., Du W., Lin H., Wang T., Subramanian C., Moon J.J., Cieslik M., Cohen M, & Zou W. (2021). LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy. Nature cell biology, 23(5), 526-537.
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2

Immunofluorescence Imaging of GBP1-5 and HSP90

Check if the same lab product or an alternative is used in the 5 most similar protocols
A375 cells mounted on cover slips were treated with IFNγ for 24 hours. After washing 2 times with PBS, cells were fixed with 4% PFA for 15 minutes and washed 2 times with PBS for 5 minutes each. Then, the cells were permeabilized with 0.5% triton x-100 in PBS for 10 minutes and rinsed 2 times with PBS for 5 minutes each. Antigens were blocked with 10% normal goal serum in PBS for 30 minutes. Then, primary antibodies were added at 1:50 dilutions of mouse anti-human GBP1-5 antibody (Santa Cruz, 166960) or rabbit anti-human HSP90 antibody (CST, 4877), and incubated at 4°C overnight. Afterwards, the cells were washed and incubated with 1:500 dilutions of Qdot 605 labeled secondary goat anti-mouse antibody (Thermo Fisher, Q11002) or AF488-labeled secondary goat anti-rabbit antibody (Thermo Fisher, A11034), and then mounted on glass slides with ProLong Gold reagent containing DAPI. Confocal fluorescence images were collected using a 63X oil-immersion objective (Leica SP5 Inverted 2-Photon FLIM confocal).
Li G., Kryczek I., Nam J., Li X., Li S., Li J., Wei S., Grove S., Vatan L., Zhou J., Du W., Lin H., Wang T., Subramanian C., Moon J.J., Cieslik M., Cohen M, & Zou W. (2021). LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy. Nature cell biology, 23(5), 526-537.
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