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2 protocols using disodium tetraborate borax

1

LC-MS Analysis of Biogenic Amines

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Liquid chromatography-mass spectrometry (LC-MS)-grade acetonitrile, hexane, and LC-MS water were provided by Witko (Łódź, Poland). Disodium tetraborate (borax) with a purity of ≥99% was sourced from Chempur (Piekary Śląskie, Poland). Ammonium formate with a purity of ≥97% and formic acid with an assay of 98 to 100% were procured from Chem-Lab (Zedelgem, Belgium). Dansyl chloride with a purity of 97% was obtained from abcr GmbH (Karlsruhe, Germany). Pure trichloroacetic acid was supplied by POCH (Gliwice, Poland). Certified analytical standards, including PUT-BAHIS-BA, CAD-BA, TRP-BA, PHM-BA, TYR-BA, SPD-BA with a purity of ≥99%, SPM-BA with a purity of ≥99%, AGM with a purity of ≥97%, 1,7-diaminoheptane with an assay of 98%, and ammonium hydroxide solute, were utilized. Additionally, β-Ala, Arg, Asp, asparagines, Cys, Ser, Orn with a purity of ≥99%, Glu, glutamine with a purity of ≥99%, His, Lys, Trp with a purity of ≥98%, Phm, Tyr, threonine, Pro, Leu, isoleucine, and Val were employed.
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2

Quantification of Biogenic Amines via LC-MS

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The LC–MS grade acetonitrile, LC–MS water, and hexane were purchased from Witko (Łódź, Poland). Di-sodium tetraborate (borax) ≥ 99% was supplied by Chempur (Piekary Śląskie, Poland). Ammonium formate ≥ 97% and formic acid assay 98–100% were purchased from Chem-Lab (Zedelgem, Belgium). Dansyl chloride 97% was purchased from abcr GmbH (Karlsruhe, Germany). Pure trichloroacetic acid was supplied by POCH (Gliwice, Poland). Certified analytical standard (agmatine ≥ 97%, putrescine, histamine, cadaverine, tryptamine, phenylethylamine, tyramine, spermidine, spermine, arginine, ornithine, glutamine ≥ 99%, histidine, lysine, tryptophan ≥ 98%, phenethylamine, tyrosine, 1,7-diamino heptane assay 98%, and ammonium hydroxide solution ~ 25%) were supplied by Sigma-Aldrich (Darmstadt, Germany). The Folin Ciocalteau reagent, DPPH, Trolox, and gallic acid, methanol, and sodium carbonate were purchased from Sigma-Aldrich.
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