The embryonic Carcinoma stem cell line (ECSCs;NT2) was cultured in DMEM (Gibco, UK) supplemented with FCS (Gibco) and 2 mM L-glutamine. Cell passage was carried out by treatment with trypsin (Gibco), and cells were seeded in new dishes at a 1:5 ratio. Neural induction of these cells was accomplished in growth medium supplemented with different inducers and factors over a three-month period. Initially, 2×104 cells/cm2 were seeded in adherent tissue culture dishes in the presence of 10 μM RA for a month. The resultant compact neuro epithelial cells were dissociated by trypsin and seeded in a 3:7 ratio in new adherent culture dishes in the presence of 1 μM cytosine arabinosin (Sigma, USA) only for the first week of this period, 10 μM fluorodeoxyuridine (Sigma, USA) and 10 μM uridine for a month. For neural maturation, the apparent cell aggregates were mechanically dissociated by hitting to the side of the tissue culture dish. Dislodged aggregates were seeded in poly-D-lysine (Sigma)-coated dishes in the presence of 1 μM cytosine ara binosin (for the first week) and 10 μM fluorodeoxyuridine (16 (link)).
Cytosine arabinosin
Manufactured by Merck Group
Sourced in United States
Cytosine arabinosin is a laboratory reagent used in biochemical and molecular biology research. It is a pyrimidine nucleoside analog that inhibits DNA synthesis. The core function of cytosine arabinosin is to serve as a tool for investigating cellular processes and DNA-related mechanisms.
Automatically generated - may contain errors
Lab products found in correlation
Fluorodeoxyuridine, by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Merck Group (1 mentions)
Poly d lysine, by Merck Group (1 mentions)
1 protocol using cytosine arabinosin
1
Neuronal Differentiation of Embryonic Carcinoma Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
The embryonic Carcinoma stem cell line (ECSCs;NT2) was cultured in DMEM (Gibco, UK) supplemented with FCS (Gibco) and 2 mM L-glutamine. Cell passage was carried out by treatment with trypsin (Gibco), and cells were seeded in new dishes at a 1:5 ratio. Neural induction of these cells was accomplished in growth medium supplemented with different inducers and factors over a three-month period. Initially, 2×104 cells/cm2 were seeded in adherent tissue culture dishes in the presence of 10 μM RA for a month. The resultant compact neuro epithelial cells were dissociated by trypsin and seeded in a 3:7 ratio in new adherent culture dishes in the presence of 1 μM cytosine arabinosin (Sigma, USA) only for the first week of this period, 10 μM fluorodeoxyuridine (Sigma, USA) and 10 μM uridine for a month. For neural maturation, the apparent cell aggregates were mechanically dissociated by hitting to the side of the tissue culture dish. Dislodged aggregates were seeded in poly-D-lysine (Sigma)-coated dishes in the presence of 1 μM cytosine ara binosin (for the first week) and 10 μM fluorodeoxyuridine (16 (link)).
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The embryonic Carcinoma stem cell line (ECSCs;NT2) was cultured in DMEM (Gibco, UK) supplemented with FCS (Gibco) and 2 mM L-glutamine. Cell passage was carried out by treatment with trypsin (Gibco), and cells were seeded in new dishes at a 1:5 ratio. Neural induction of these cells was accomplished in growth medium supplemented with different inducers and factors over a three-month period. Initially, 2×104 cells/cm2 were seeded in adherent tissue culture dishes in the presence of 10 μM RA for a month. The resultant compact neuro epithelial cells were dissociated by trypsin and seeded in a 3:7 ratio in new adherent culture dishes in the presence of 1 μM cytosine arabinosin (Sigma, USA) only for the first week of this period, 10 μM fluorodeoxyuridine (Sigma, USA) and 10 μM uridine for a month. For neural maturation, the apparent cell aggregates were mechanically dissociated by hitting to the side of the tissue culture dish. Dislodged aggregates were seeded in poly-D-lysine (Sigma)-coated dishes in the presence of 1 μM cytosine ara binosin (for the first week) and 10 μM fluorodeoxyuridine (16 (link)).
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