Ly6c vioblue

Manufactured by Miltenyi Biotec

Ly6C-Vioblue is a fluorochrome-conjugated antibody that recognizes the Ly6C antigen, which is expressed on various cell types. The core function of this product is to facilitate the identification and analysis of Ly6C-positive cells through flow cytometry or other applicable techniques.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using ly6c vioblue

Multicolor Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibody reagents were obtained from Miltenyi Biotech: REA clones of antimouse CD3-APC-Vio770 (REA606), CD4-Vioblue (REA 604), CD8a-PE-Vio770 (REA 601), Foxp3-Vio515 (REA 788), NKp46-APC (REA 815), CD11c-PE (REA 754), CD11b-APC-Vio770 (REA 592), Ly6C-Vioblue (REA 796), Ly6G-PE-Vio770 (REA 526). Foxp3 staining was performed using Foxp3 Staining Buffer Set (Miltenyi Biotech).
Flow cytometry analysis was performed using a Miltenyi 8-color MACSQuant, and data were analyzed using Flowlogic (Miltenyi Biotech).

Lantier L., Poupée-Beaugé A., di Tommaso A., Ducournau C., Epardaud M., Lakhrif Z., Germon S., Debierre-Grockiego F., Mévélec M.N., Battistoni A., Coënon L., Deluce-Kakwata-Nkor N., Velge-Roussel F., Beauvillain C., Baranek T., Lee G.S., Kervarrec T., Touzé A., Moiré N, & Dimier-Poisson I. (2020). Neospora caninum: a new class of biopharmaceuticals in the therapeutic arsenal against cancer. Journal for Immunotherapy of Cancer, 8(2), e001242.

+ Open protocol

+ Expand

Isolation of Murine Cardiac Myeloid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

LV tissue excised from day 0 and day 1 post-MI mice was minced and digested with 600 U/ml collagenase II (Worthington, LS004177, Lot 47E17554B) and 60 U/ml DNase I in Hanks buffered saline solution and filtered through a 30-µm separation filter to generate single-cell suspensions. Red blood cells were lysed (Red Blood Cell Lysis Solution, Miltenyi 130-094-183) and non-specific interactions were blocked with FcR Blocking Reagent (Miltenyi 130-092-575). Cells were stained with the following fluorophore-conjugated antibody panels: CD45-FITC (Miltenyi 130-110-658), CD11b-APC-Vio770^® (Miltenyi 130-109-288), F4/80-PerCP-Vio700 (Miltenyi 130-102-161), Ly6C-VioBlue^® (Miltenyi 130-111-921), and Ly6G-APC (Miltenyi 130-107-914). Samples were quantified using the MACSQuant Analyzer 10 (Miltenyi). Cell populations were gated on live singlets, with cells from monocyte-derived/macrophage lineage classified as CD45⁺CD11b⁺Ly6G⁻ cells.

Mouton A.J., DeLeon-Pennell K.Y., Rivera Gonzalez O.J., Flynn E.R., Freeman T.C., Saucerman J.J., Garrett M.R., Ma Y., Harmancey R, & Lindsey M.L. (2018). Mapping macrophage polarization over the myocardial infarction time continuum. Basic Research in Cardiology, 113(4), 26.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!