In total, 5-ml venous blood samples were withdrawn by EDTA into K2-containing BD vacutainers (purple-capped; Becton-Dickinson, Franklin Lakes, NJ, USA) from the NSCLC patients and healthy control subjects. The samples were concentrated using a Jumbosep™ Centrifugal Device (Pall Corporation, Port Washington, NY, USA) at 4,300.8 × g for 15 min, and the plasma was separated and stored at <−20°C until analysis was performed. Additionally, ELISA was used to assess the VEGF 165 plasma levels.
Jumbosep centrifugal device
Manufactured by Pall Corporation
Sourced in United States
The Jumbosep™ Centrifugal Device is a laboratory equipment product offered by Pall Corporation. It is a high-capacity centrifugal device designed for separation and concentration applications.
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Lab products found in correlation
2 protocols using jumbosep centrifugal device
1
VEGF 165 Plasma Level Analysis
2
Sample Concentration for Downstream Analysis
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A total of 120 mL of the sample was first concentrated, at 3000g for 30 min by loading 60 mL of the sample twice, to approximately 5 mL using Jumbosep Centrifugal Device, 30-kDa (Pall Corporation, Ann Harbor, MI, USA). Then, the 5 mL concentrate was further concentrated down at 3000g for 30 min using Microsep Advance Centrifugal Device, 30-kDa, (Pall Corporation, Ann Harbor, MI, USA). The final volume of the concentrate varied between 500 and 1200 μL.
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