The reference standards for lapatinib (≥98% purity) and dasatinib (≥98% purity) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The lapatinib ditosylate tablets (250 mg per tablet) used for the animal experiments were produced by Glaxo Operations UK Limited (Ware, Herts, UK). TPF-T was produced by FRESENIUS KABI SSPC (Wuxi, China), with caloric distribution of 18% protein, 50% fat, and 32% carbohydrate. Detailed nutrient formulation of TPF-T can be found in the Supplementary materials (Table S1 ). Formic acid was purchased from Aladdin (Shanghai, China), whereas HPLC-grade methanol and acetonitrile were obtained from Merck (Darmstadt, Germany). Ultrapure water was prepared using a Milli-Q A10 water purification system (Millipore, Billerica, MA, USA).
Milli q a10 water purification system
Manufactured by Merck Group
Sourced in United States
The Milli-Q A10 water purification system is a compact and reliable instrument designed to produce ultrapure water for a variety of laboratory applications. The system utilizes a combination of advanced purification technologies, including reverse osmosis and electrodeionization, to remove a wide range of impurities from the water supply, ensuring consistent and high-quality water output.
Automatically generated - may contain errors
Lab products found in correlation
Glacial acetic acid, by Merck Group (3 mentions)
Caffeic acid, by LGC (3 mentions)
Rosmarinic acid, by LGC (3 mentions)
Carnosic acid, by LGC (3 mentions)
Ursolic acid, by LGC (3 mentions)
Carnosol, by Cayman Chemical (3 mentions)
Lc ms grade methanol, by Honeywell (2 mentions)
Lc ms grade formic acid, by Merck Group (2 mentions)
Lc ms grade acetonitrile, by Honeywell (2 mentions)
Luteolin 7 glucoside, by LGC (2 mentions)
13c caffeic acid, by Cayman Chemical (2 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Dasatinib, by Merck Group (1 mentions)
Lapatinib, by Merck Group (1 mentions)
Tripletof 5600, by AB Sciex (1 mentions)
Chlorogenic acid, by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Apigenin, by Merck Group (1 mentions)
Luteolin, by Merck Group (1 mentions)
5 protocols using milli q a10 water purification system
1
Quantification of Lapatinib and Dasatinib
2
Gravimetric Preparation of Reference Materials
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High-density polyethylene (HDPE) containers with a maximum volume of 8 L and glass containers with a maximum volume of 11 L were used to prepare RM batches for PT-1 and PT-2, respectively. 125 mL HDPE and 250 mL polypropylene bottles were used as containers for all the prepared RMs. Aluminium foil zip lock bags were used for packaging and to avoid solvent transpiration from the prepared RMs. Model XPE6003SD5 (Mettler Toledo, Switzerland), CCE60K3 (Sartorius, Germany) and CCE1005 (Sartorius, Germany) balances were used for gravimetric preparation of batches and gravimetric dilutions of the standards solutions. Finally, a Milli-Q A10 water purification system (Millipore, Paris, France) was used to supply the high purity type I water. A summary of the gravimetric preparation of the RM batches is shown in Supplementary Table S2 .
3
Comprehensive Phytochemical Profiling by LC-MS
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LCMS grade methanol and acetonitrile were procured from Honeywell and formic acid and glacial acetic acid were purchased from Merck. Reference standards, apigenin, luteolin, rutin, chlorogenic acid, hesperidin, and hesperetin were obtained from Sigma-Aldrich. Caffeic acid, carnosic acid, luteolin-7-O-glucoside, ursolic acid, and rosmarinic acid were procured from Toronto Research Chemicals. Narirutin and carnosol were from Cayman Chemical. Ultrapure water produced using a Milli-Q A10 water purification system (Millipore Sigma) was used throughout. Mass spectrometry was performed using a Triple TOF-5600 (AB SCIEX), a hybrid triple QTOF mass spectrometer equipped with an ESI source, with the mass range set at m/z 50-1000. ESI was set in the negative mode with an ion spray needle voltage set at 4500 V and ion source gas-1 and gas-2 each set at 50 psi. The curtain gas was 35 psi and the collision gas pressure was 20 psi with a source temperature of 600 °C. The declustering potential was -50 eV in TOF-MS and MS/MS experiments, whereas the collision energy values for TOF-MS were 5 eV, and for MS/MS experiments, 25 eV with a spread of 15 eV.
4
LCMS Characterization of Phenolic Compounds
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LCMS grade acetonitrile and methanol were purchased from Honeywell, Burdick, and Jackson, USA. LCMS grade formic acid and glacial acetic acid were procured from Merck, Germany. Caffeic acid, rosmarinic acid, carnosic acid, ursolic acid, and luteolin 7-glucoside were purchased from Toronto Research Chemicals, Canada. Carnosol and 13C- Caffeic acid were purchased from Cayman Chemical, USA. Ultrapure water from the Milli-Q, A10 water purification system (Millipore Sigma, Madison, WI, USA) was used throughout the experiment.
5
LCMS Analysis of Phenolic Compounds
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LCMS grade acetonitrile was purchased from Honeywell, Burdick and Jackson, USA, and LCMS grade methanol was purchased from Honeywell, Riedel-de-Haen, USA. LCMS grade formic acid and glacial acetic acid were procured from Merck, Germany. Caffeic acid, rosmarinic acid, carnosic acid, ursolic acid, and luteolin-7-glucoside were purchased from Toronto Research Chemicals, Canada. Carnosol and 13 C-Caffeic acid were purchased from Cayman Chemical, USA. Ultrapure water produced using a Milli-Q; A10 water purification system (Millipore Sigma, USA) was used throughout the experiment.
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